Nanocs SnapLink Biotin hydrazide is a hydrazide-activated biotinylation reagent for labeling glycoproteins and other carbohydrate-containing molecules having oxidizable sugars or aldehydes.
Nanocs SnapLink Biotin PEG hydrazide is a pegylated biotinylation reagents for labeling glycoproteins or other carbohydrate-containing molecules. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the biolinylated molecules , thus reducing aggregation of labeled proteins in solution. The PEG spacer arm also gives the biotin a long and flexible structure to minimize steric hindrance for binding to streptavidin or avdins.
These biotin hydrazide reagents enable simple and efficient biotin labeling of polyclonal antibodies and other glycoproteins. Mild oxidation of antibodies with sodium periodate produces reactive aldehydes on the carbohydrate moieties of the Fc portion that can be modified by hydrazides. This approach is advantageous for labeling antibodies because biotinylation occurs only at the sites of glycosylation, which are primarily in the Fc region of the antibody, far from the antigen binding site.
- Glycoprotein labelingBiotinylate glycosylated proteins at sialic acid residues for detection or purification using streptavidin probes or resins
- Cell surface labelingBiotinylate and isolate cell surface glycoproteins
- Aldehyde-reactive reacts with aldehydes formed by periodate-oxidation of sugar groups
- Hydrazide-activatedperform reactions at pH 4 to 6 in buffers such as sodium acetate
- Irreversible forms semi-permanent hydrazone bonds; spacer arm cannot be cleaved
- Appearance (color): White to off-white.
- Appearance (form): Semi-solid to solid;
- Solubility: Soluble in DMSO, DMF;
- Store-20 0C, dessiccate. Protect from light.