Nanocs SnapLink PFP PEG biotin (Pentafluorophenyl PEG Biotin) is a pegylated, amine reactive biotinylation reagent similar to Biotin-PEG-NHS. Compare to NHS-PEG-Biotin, PFP-PEG-Biotin is more reactive and it can be used to label both primary and secondary amine groups in pH 7 to 9. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the biotinylated molecules , thus reducing aggregation of labeled substrates in solution. The PEG spacer arm also gives the biotin a long and flexible structure to minimize steric hindrance for binding to streptavidin or avdins. Pentafluorophenyl esters of biotin react efficiently with primary and secondary amino groups (-NH2) in alkaline buffer to form a stable amide bond. Nanocs provides a variety of high purity biotin and its derivatives for various biotinylation and bioassay applications. These functional biotin products were purified by chromatography to remove impurities and ensure adequate applications both in-vitro and in-vivo.
- Appearance (color): White to off-white.
- Appearance (form): Semi-solid to solid;
- Solubility: Soluble in DMSO, DMF, water;
- Store-20 0C, dessiccate. Protect from light.