Thiol PEGylation

Thiol PEGylation is specific for free thiols on biological reagents. Utopbio offers some PEGylation reagents for covalent modification such as  mPEG-MAL (forms a physiological stable linkage) and mPEG-OPSS and mPEG-SH (each forms a disulfide bond to the conjugate). Cysteine is less abundant in proteins and peptides; thus, reducing both the chance for protein deactivation and the formation of PEGmers. In addition, site specific modification (point mutation) of a cysteine is an option for site-specific PEGylation. Cysteines engineered into a protein by site-directed mutagenesis, followed by PEGylation, have been demonstrated in animal models to enhance the regeneration of
injured sciatic nerves by altering both the PK and PD properties of the protein.

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