Nanocs SnapLinkTM pegylated succinimidyl cyclohexane Maleimide (SMCC) is an amine and sulfhydryl reactive crosslinker. This crosslinker is non-cleavable and membrane permeable. It contains an amine reactive NHS ester and a sulfhydryl reactive maleimide group bridged with PEG arm. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the crosslinked molecules , thus reducing aggregation of labeled proteins in solution. The PEG spacer arm also gives the maleimide NHS a long and flexible structure to minimize steric hindrance for its reaction to other molecues. Compared to other maleimide NHS ester, SMCC has higher stability in aqueous solution because of its cyclohexane bridge. NHS esters react with primary amines at pH 7-9 while the maleimide group reacts with available thiol or sulfhydryl at pH 6.5~7.5 to form stable thioether bonds.
- Appearance: Off-white/brownish solid or semi-solid;
- Reactivity: Reactive to sulfhydryl and amine groups;
- Solubility: Soluble in DMF or DMSO;
- Store at -20 0C, desiccated. Avoid frequent thaw and freeze.