Nanocs SnapLink TMmaleimide PEG NHS is a pegylated, amine and sulfhydryl reactive cross-linker. This cross-linker is non-cleavable and water soluble. It contains an amine reactive, NHS ester and a sulfhydryl reactive maleimide group linked with polyethylene glycol of different length. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the crosslinked molecules , thus reducing aggregation of labeled proteins in solution. The PEG spacer arm also gives the maleimide NHS a long and flexible structure to minimize steric hindrance for its reaction to other molecues. NHS esters react with deprotonated primary amines in pH 7-9. The maleimide group reacts with thiol groups at pH 6.5~7.5 to form stable thioether bonds.
- Appearance: Off-white semi-solid or solid;
- Reactivity: Reactive to sulfhydryl and amine groups;
- Solubility: Soluble in DMSO or DMF.
- Store at -20 0C, desiccated. Avoid frequent thaw and freeze.