DODAP (1,2-dioleoyl-3-dimethylammonium-propane) is an ionizable cationic lipid with low cytotoxicity and high transfection efficiency. DODAP is neutral at physiological pH, but acquires a positive charge inside the endosome due to the protonation of free amines when pH is lower than its pKa (<7). The electrostatic interactions between DODAP and naturally occurring anionic lipids in endosomal membranes trigger the release of nucleic acid. These interactions promote membrane lytic non-bilayer structures to enable the intracellular delivery of nucleic acid. A common application is in nanomedicine as an ionizable lipid component of nanocarriers (lipid-polymer hybrid nanoparticles, LPNs), widely used to encapsulate bioactive molecules, including mRNA, siRNA, and plasmid DNA as a treatment for diseases.
- Form: Liquid;
- Color: Light yellow liquid;
- Formular: C41H77NO4;
- Molecular weight: 648.05;
- Lipid type: C18, un-saturated ionizable lipids;
- DNA, SiRNA, mRNA transfection;
- Drug delivery;
- Gene therapy;
- Store at -20 0C. Protect from light.
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