Hydrazide PEG DSPE

FITC PEG hydrazide

Hydrazide PEG FITC

Maleimide PEG hydrazide

Hydrazide PEG Maleimide

PDP PEG Hydrazide

Hydrazide PEG PDP

Thiol PEG Hydrazide

Hydrazide PEG Thiol

Tetrazine PEG Hydrazide

Tetrazine PEG Hydrazide

DSPE PEG Aldehyde,DSPE-PEG-CHO

Synonym: 1,2-distearoyl-sn-glycero-3-phosphoethanolamine PEG aldehyde, DSPE-PEG-CHO, Aldehyde PEG DSPE

Description:

DSPE PEG aldehyde (DSPE-PEG-CHO) is one of Nanocs’ reactive phospholipid PEG derivatives that can react with N-terminus amine groups. DSPE (1,2-distearoyl-sn-glycero-3-phosphoethanolamine) is an 18 carbon phospholipid that is highly hydrophobic. PEG backbone, on the other hand, offers good hydrophilicity. Aldehyde PEG DSPE is a reactive lipid PEG with good reactivity towards amine, oxyamine or hydrazide groups. Aldehyde reacts with N-terminus amine groups to form an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. Aldehyde PEG DSPE allows conjugation of DSPE PEG to amine or hydrazide group at acidic conditions with good selectivity. Higher pH will result in multiple pegylation with amine groups.

Products Information:

• Form: Solid;
• Color: White;
• Reactive group: Aldehyde (-CHO)
• Reactive to: N-terminus amine
• Purity: >90%
• Solubility: Soluble in warm water;

Storage Conditions:

• Store at -20 ⁰C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
• PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

DSPE PEG Aldehyde

Synonym: 1,2-distearoyl-sn-glycero-3-phosphoethanolamine PEG aldehyde, DSPE-PEG-CHO, Aldehyde PEG DSPE

Description:

DSPE PEG aldehyde (DSPE-PEG-CHO) is one of Nanocs’ reactive phospholipid PEG derivatives that can react with N-terminus amine groups. DSPE (1,2-distearoyl-sn-glycero-3-phosphoethanolamine) is an 18 carbon phospholipid that is highly hydrophobic. PEG backbone, on the other hand, offers good hydrophilicity. Aldehyde PEG DSPE is a reactive lipid PEG with good reactivity towards amine, oxyamine or hydrazide groups. Aldehyde reacts with N-terminus amine groups to form an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. Aldehyde PEG DSPE allows conjugation of DSPE PEG to amine or hydrazide group at acidic conditions with good selectivity. Higher pH will result in multiple pegylation with amine groups.

Products Information:

• Form: Solid;
• Color: White;
• Reactive group: Aldehyde (-CHO)
• Reactive to: N-terminus amine
• Purity: >90%
• Solubility: Soluble in warm water;

Storage Conditions:

• Store at -20 ⁰C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
• PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

DSPE PEG Aldehyde

Synonym: 1,2-distearoyl-sn-glycero-3-phosphoethanolamine PEG aldehyde, DSPE-PEG-CHO, Aldehyde PEG DSPE

Description:

DSPE PEG aldehyde (DSPE-PEG-CHO) is one of Nanocs’ reactive phospholipid PEG derivatives that can react with N-terminus amine groups. DSPE (1,2-distearoyl-sn-glycero-3-phosphoethanolamine) is an 18 carbon phospholipid that is highly hydrophobic. PEG backbone, on the other hand, offers good hydrophilicity. Aldehyde PEG DSPE is a reactive lipid PEG with good reactivity towards amine, oxyamine or hydrazide groups. Aldehyde reacts with N-terminus amine groups to form an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. Aldehyde PEG DSPE allows conjugation of DSPE PEG to amine or hydrazide group at acidic conditions with good selectivity. Higher pH will result in multiple pegylation with amine groups.

Products Information:

• Form: Solid;
• Color: White;
• Reactive group: Aldehyde (-CHO)
• Reactive to: N-terminus amine
• Purity: >90%
• Solubility: Soluble in warm water;

Storage Conditions:

• Store at -20 ⁰C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
• PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Cholesterol PEG aldehyde, CLS-PEG-CHO

Description:

Nanocs Cholesterol PEG aldehyde is a lipid PEG derivative with amine reactive aldehyde group. Aldeyde PEG is a common N-terminal amine group pegylation reagent. The reaction between aldehyde group with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.

Physical Properties:

• Off-white/white solid;
• Soluble in warm water, DMSO, DMF;

Storage Conditions:

• Store at -20 ⁰C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
• PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Cholesterol PEG aldehyde, CLS-PEG-CHO

Description:

Nanocs Cholesterol PEG aldehyde is a lipid PEG derivative with amine reactive aldehyde group. Aldeyde PEG is a common N-terminal amine group pegylation reagent. The reaction between aldehyde group with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.

Physical Properties:

• Off-white/white solid;
• Soluble in warm water, DMSO, DMF;

Storage Conditions:

• Store at -20 ⁰C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
• PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Cholesterol PEG aldehyde, CLS-PEG-CHO

Description:

Nanocs Cholesterol PEG aldehyde is a lipid PEG derivative with amine reactive aldehyde group. Aldeyde PEG is a common N-terminal amine group pegylation reagent. The reaction between aldehyde group with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.

Physical Properties:

• Off-white/white solid;
• Soluble in warm water, DMSO, DMF;

Storage Conditions:

• Store at -20 ⁰C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
• PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Stearic acid PEG aldehyde

Description:

Nanocs Stearic acid PEG aldehyde is an 18C fatty acid lipid PEG derivative with amine reactive aldehyde group. Aldeyde PEG is a common N-terminal amine groupation reagent. The reaction between aldehyde group with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.

Physical Properties:

• Off-white/white solid;
• Soluble in warm water, DMSO, DMF;

Storage Conditions:

• Store at -20 ⁰C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
• PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Stearic acid PEG aldehyde

Description:

Nanocs Stearic acid PEG aldehyde is an 18C fatty acid lipid PEG derivative with amine reactive aldehyde group. Aldeyde PEG is a common N-terminal amine groupation reagent. The reaction between aldehyde group with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.

Physical Properties:

• Off-white/white solid;
• Soluble in warm water, DMSO, DMF;

Storage Conditions:

• Store at -20 ⁰C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
• PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Stearic acid PEG aldehyde

Description:

Nanocs Stearic acid PEG aldehyde is an 18C fatty acid lipid PEG derivative with amine reactive aldehyde group. Aldeyde PEG is a common N-terminal amine groupation reagent. The reaction between aldehyde group with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.

Physical Properties:

• Off-white/white solid;
• Soluble in warm water, DMSO, DMF;

Storage Conditions:

• Store at -20 ⁰C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
• PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Fluorescein PEG aldehyde

Fluorescein PEG Aldehyde, FITC-PEG-CHO

Fluorescein PEG Aldehde (FITC-PEG-CHO) is one of Nanocs’ reactive fluorescent PEG dyes that can react with N-terminal amines. Fluorescein is a bright green fluorescent dye with excitation/emission wavelength at 495 nm/515 nm. Aldehyde group is reactive toward amines, hydrazide or oxyamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with N-terminal amine groups at acidic pH, a condition sometimes required for certain bioconjugation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldhyde and other groups allows site-specific conjugation and labeling of fuorescein dyes to desired position on targeted molecules. Conjugated FITC dye can be easily detected by common fluorescent instruments under FITC channel. PEG linker between FITC and aldehyde offers higher water solubility, less steric hindrance and enhanced stability. Compared to other commonly used fluorescent dyes, Nanocs pegylated fluorescent dyes are more stable and easier to use.

Physical Properties:

• Appearance: Orange/yellow solid;
• Solubility: Soluble in water, DMSO, DMF;
• Reactive group: Aldehyde (-CHO);
• Reactive toward: N-terminal amines, primary amine, aminooxy, hydrazide, etc.;
• Ex/Em wavelength: 495 nm/515 nm;

Storage Conditions:

• Store at -20 ⁰C. Protect from light.

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of Fluorescein PEG aldehyde over the amount of amine-containing molecules (such as protein or antibody) results in sufficient conjugation.

Materials Required:

• Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
• FITC PEG aldehyde stock solution: 5-10 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted moleculess in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add FITC PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Fluorescein PEG aldehyde

Fluorescein PEG Aldehyde, FITC-PEG-CHO

Fluorescein PEG Aldehde (FITC-PEG-CHO) is one of Nanocs’ reactive fluorescent PEG dyes that can react with N-terminal amines. Fluorescein is a bright green fluorescent dye with excitation/emission wavelength at 495 nm/515 nm. Aldehyde group is reactive toward amines, hydrazide or oxyamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with N-terminal amine groups at acidic pH, a condition sometimes required for certain bioconjugation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldhyde and other groups allows site-specific conjugation and labeling of fuorescein dyes to desired position on targeted molecules. Conjugated FITC dye can be easily detected by common fluorescent instruments under FITC channel. PEG linker between FITC and aldehyde offers higher water solubility, less steric hindrance and enhanced stability. Compared to other commonly used fluorescent dyes, Nanocs pegylated fluorescent dyes are more stable and easier to use.

Physical Properties:

• Appearance: Orange/yellow solid;
• Solubility: Soluble in water, DMSO, DMF;
• Reactive group: Aldehyde (-CHO);
• Reactive toward: N-terminal amines, primary amine, aminooxy, hydrazide, etc.;
• Ex/Em wavelength: 495 nm/515 nm;

Storage Conditions:

• Store at -20 ⁰C. Protect from light.

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of Fluorescein PEG aldehyde over the amount of amine-containing molecules (such as protein or antibody) results in sufficient conjugation.

Materials Required:

• Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
• FITC PEG aldehyde stock solution: 5-10 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted moleculess in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add FITC PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Fluorescein PEG aldehyde

Fluorescein PEG Aldehyde, FITC-PEG-CHO

Fluorescein PEG Aldehde (FITC-PEG-CHO) is one of Nanocs’ reactive fluorescent PEG dyes that can react with N-terminal amines. Fluorescein is a bright green fluorescent dye with excitation/emission wavelength at 495 nm/515 nm. Aldehyde group is reactive toward amines, hydrazide or oxyamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with N-terminal amine groups at acidic pH, a condition sometimes required for certain bioconjugation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldhyde and other groups allows site-specific conjugation and labeling of fuorescein dyes to desired position on targeted molecules. Conjugated FITC dye can be easily detected by common fluorescent instruments under FITC channel. PEG linker between FITC and aldehyde offers higher water solubility, less steric hindrance and enhanced stability. Compared to other commonly used fluorescent dyes, Nanocs pegylated fluorescent dyes are more stable and easier to use.

Physical Properties:

• Appearance: Orange/yellow solid;
• Solubility: Soluble in water, DMSO, DMF;
• Reactive group: Aldehyde (-CHO);
• Reactive toward: N-terminal amines, primary amine, aminooxy, hydrazide, etc.;
• Ex/Em wavelength: 495 nm/515 nm;

Storage Conditions:

• Store at -20 ⁰C. Protect from light.

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of Fluorescein PEG aldehyde over the amount of amine-containing molecules (such as protein or antibody) results in sufficient conjugation.

Materials Required:

• Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
• FITC PEG aldehyde stock solution: 5-10 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted moleculess in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add FITC PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Biotin PEG aldehyde

Biotin PEG Aldehyde

Biotin PEG Aldehde (Biotin-PEG-CHO) is one of Nanocs’ chemical reactive biotin PEG derivatives that can label N-terminal amines. Aldehyde group is reactive toward amine, hydrazide or oxyamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with protonated amines at acidic environment (pH ~4), a condition sometimes required for certain site-specific biotinylation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldhyde and other groups allows site-specific conjugation and labeling of biotin tag to desired position on targeted molecules. Resulted biotin can recognize and bind to avidin, streptavidin or neutravidin with high affinity. PEG linker between Biotin and aldehyde offers higher water solubility, less steric hindrance and enhanced stability. Compared to other commonly used biotinylation reagents, Nanocs pegylated biotins are water soluble, more stable and easier to use.

Physical Properties:

• Appearance: White/off-whilte solid;
• Solubility: Soluble in water, DMSO, DMF;
• Reacive group: Aldehyde (-CHO);
• Reactive to: N-terminal amine, primary amine, etc.;
• Functional moiety: D-biotin;
• Reactive toward: avidin, streptavidin or neutravidin;

Storage Conditions:

• Store at -20 ⁰C. Desiccate.

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of Biotin PEG aldehyde over the amount of amine-containing molecules (such as protein or antibody) results in sufficient conjugation.

Materials Required:

• Reaction buffer: Amine-free aqueous buffer, pH 5.0~6.5.
• Biotin PEG aldehyde stock solution: 5-10 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve ready labeling molecules in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add Biotin PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Biotin PEG aldehyde, Aldehyde PEG Biotin

Biotin PEG Aldehyde

Biotin PEG Aldehde (Biotin-PEG-CHO) is one of Nanocs’ chemical reactive biotin PEG derivatives that can label N-terminal amines. Aldehyde group is reactive toward amine, hydrazide or oxyamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with protonated amines at acidic environment (pH ~4), a condition sometimes required for certain site-specific biotinylation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldhyde and other groups allows site-specific conjugation and labeling of biotin tag to desired position on targeted molecules. Resulted biotin can recognize and bind to avidin, streptavidin or neutravidin with high affinity. PEG linker between Biotin and aldehyde offers higher water solubility, less steric hindrance and enhanced stability. Compared to other commonly used biotinylation reagents, Nanocs pegylated biotins are water soluble, more stable and easier to use.

Physical Properties:

• Appearance: White/off-whilte solid;
• Solubility: Soluble in water, DMSO, DMF;
• Reacive group: Aldehyde (-CHO);
• Reactive to: N-terminal amine, primary amine, etc.;
• Functional moiety: D-biotin;
• Reactive toward: avidin, streptavidin or neutravidin;

Storage Conditions:

• Store at -20 ⁰C. Desiccate.

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of Biotin PEG aldehyde over the amount of amine-containing molecules (such as protein or antibody) results in sufficient conjugation.

Materials Required:

• Reaction buffer: Amine-free aqueous buffer, pH 5.0~6.5.
• Biotin PEG aldehyde stock solution: 5-10 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve ready labeling molecules in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add Biotin PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Biotin PEG aldehyde, Aldehyde PEG Biotin

Biotin PEG Aldehyde

Biotin PEG Aldehde (Biotin-PEG-CHO) is one of Nanocs’ chemical reactive biotin PEG derivatives that can label N-terminal amines. Aldehyde group is reactive toward amine, hydrazide or oxyamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with protonated amines at acidic environment (pH ~4), a condition sometimes required for certain site-specific biotinylation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldhyde and other groups allows site-specific conjugation and labeling of biotin tag to desired position on targeted molecules. Resulted biotin can recognize and bind to avidin, streptavidin or neutravidin with high affinity. PEG linker between Biotin and aldehyde offers higher water solubility, less steric hindrance and enhanced stability. Compared to other commonly used biotinylation reagents, Nanocs pegylated biotins are water soluble, more stable and easier to use.

Physical Properties:

• Appearance: White/off-whilte solid;
• Solubility: Soluble in water, DMSO, DMF;
• Reacive group: Aldehyde (-CHO);
• Reactive to: N-terminal amine, primary amine, etc.;
• Functional moiety: D-biotin;
• Reactive toward: avidin, streptavidin or neutravidin;

Storage Conditions:

• Store at -20 ⁰C. Desiccate.

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of Biotin PEG aldehyde over the amount of amine-containing molecules (such as protein or antibody) results in sufficient conjugation.

Materials Required:

• Reaction buffer: Amine-free aqueous buffer, pH 5.0~6.5.
• Biotin PEG aldehyde stock solution: 5-10 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve ready labeling molecules in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add Biotin PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Maleimide PEG aldehyde

Description:

Maleimide PEG aldehyde is one of Nanocs premier thiol (-SH) and N-terminal amine group pegylation reagents. Maleimide group reacts with sulfhydryl (thiol) group at pH 6.5-7.5 rapidly and specifically while aldehyde group reacts with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. Pegylated maleimide and aldehydes are water soluble and all reactions can be carried out in aqueous buffer. Aldehyde reacts with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.

Physical Properties:

• White/off-white solid;
• Soluble in warm water, DMSO, DMF;

Storage Conditions:

• Store at -20 ⁰C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of Maleimide PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Pegylation buffer: Amine and thiol free aqueous buffer, pH 5.0~6.5.
• Maleimide PEG aldehyde stock solution: 20 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add maleimide PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Maleimide PEG aldehyde

Description:

Maleimide PEG aldehyde is one of Nanocs premier thiol (-SH) and N-terminal amine group pegylation reagents. Maleimide group reacts with sulfhydryl (thiol) group at pH 6.5-7.5 rapidly and specifically while aldehyde group reacts with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. Pegylated maleimide and aldehydes are water soluble and all reactions can be carried out in aqueous buffer. Aldehyde reacts with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.

Physical Properties:

• White/off-white solid;
• Soluble in warm water, DMSO, DMF;

Storage Conditions:

• Store at -20 ⁰C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of Maleimide PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Pegylation buffer: Amine and thiol free aqueous buffer, pH 5.0~6.5.
• Maleimide PEG aldehyde stock solution: 20 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add maleimide PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Maleimide PEG aldehyde

Description:

Maleimide PEG aldehyde is one of Nanocs premier thiol (-SH) and N-terminal amine group pegylation reagents. Maleimide group reacts with sulfhydryl (thiol) group at pH 6.5-7.5 rapidly and specifically while aldehyde group reacts with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. Pegylated maleimide and aldehydes are water soluble and all reactions can be carried out in aqueous buffer. Aldehyde reacts with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.

Physical Properties:

• White/off-white solid;
• Soluble in warm water, DMSO, DMF;

Storage Conditions:

• Store at -20 ⁰C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of Maleimide PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Pegylation buffer: Amine and thiol free aqueous buffer, pH 5.0~6.5.
• Maleimide PEG aldehyde stock solution: 20 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add maleimide PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

mPEG amide acetaldehyde

Description:

mPEG amide acetaldehyde is a site specific Pegylation reagent with amine reactive aldehyde group. Aldeyde PEG is a common N-terminal amine group pegylation reagent. The reaction between aldehyde group with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.

Physical Properties:

• Off-white/white solid;
• Soluble in water, DMSO, DMF;
• Activity >90%; Peroxide<10ppm;

Storage Conditions:

• Store at -20 ⁰C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
• PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

mPEG amide acetaldehyde

Description:

mPEG amide acetaldehyde is a site specific Pegylation reagent with amine reactive aldehyde group. Aldeyde PEG is a common N-terminal amine group pegylation reagent. The reaction between aldehyde group with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.

Physical Properties:

• Off-white/white solid;
• Soluble in water, DMSO, DMF;
• Activity >90%; Peroxide<10ppm;

Storage Conditions:

• Store at -20 ⁰C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
• PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

mPEG amide acetaldehyde

Description:

mPEG amide acetaldehyde is a site specific Pegylation reagent with amine reactive aldehyde group. Aldeyde PEG is a common N-terminal amine group pegylation reagent. The reaction between aldehyde group with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.

Physical Properties:

• Off-white/white solid;
• Soluble in water, DMSO, DMF;
• Activity >90%; Peroxide<10ppm;

Storage Conditions:

• Store at -20 ⁰C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
• PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

mPEG amide acetaldehyde

Description:

mPEG amide acetaldehyde is a site specific Pegylation reagent with amine reactive aldehyde group. Aldeyde PEG is a common N-terminal amine group pegylation reagent. The reaction between aldehyde group with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.

Physical Properties:

• Off-white/white solid;
• Soluble in water, DMSO, DMF;
• Activity >90%; Peroxide<10ppm;

Storage Conditions:

• Store at -20 ⁰C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
• PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

mPEG propionaldehyde

Methoxy PEG propionaldehyde is a stable aldehyde PEG that can be used for protein, antibody, peptides or carbohydrate N-terminal amine group pegylation reagent. The reaction between aldehyde group with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.

Physical Properties:

• Off-white/white solid;
• Soluble in water, DMSO, DMF;
• Activity >90%; Peroxide<10ppm;

Storage Conditions:

• Store at -20 ⁰C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
• PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

mPEG propionaldehyde

Methoxy PEG propionaldehyde is a stable aldehyde PEG that can be used for protein, antibody, peptides or carbohydrate N-terminal amine group pegylation reagent. The reaction between aldehyde group with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.

Physical Properties:

• Off-white/white solid;
• Soluble in water, DMSO, DMF;
• Activity >90%; Peroxide<10ppm;

Storage Conditions:

• Store at -20 ⁰C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
• PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

mPEG propionaldehyde

Methoxy PEG propionaldehyde is a stable aldehyde PEG that can be used for protein, antibody, peptides or carbohydrate N-terminal amine group pegylation reagent. The reaction between aldehyde group with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.

Physical Properties:

• Off-white/white solid;
• Soluble in water, DMSO, DMF;
• Activity >90%; Peroxide<10ppm;

Storage Conditions:

• Store at -20 ⁰C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
• PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

mPEG propionaldehyde

Methoxy PEG propionaldehyde is a stable aldehyde PEG that can be used for protein, antibody, peptides or carbohydrate N-terminal amine group pegylation reagent. The reaction between aldehyde group with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.

Physical Properties:

• Off-white/white solid;
• Soluble in water, DMSO, DMF;
• Activity >90%; Peroxide<10ppm;

Storage Conditions:

• Store at -20 ⁰C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
• PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

mPEG benzaldehyde

Description:

mPEG benzaldehyde is a monofunctional, site specific PEG derivative with amine reactive aldehyde group. Aldeyde PEG is a common N-terminal amine group pegylation reagent. The reaction between aldehyde group with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups. Aromatic aldehyde PEG shows better stability in aqueous solution.

Physical Properties:

• Off-white/white solid;
• Soluble in water, DMSO, DMF;
• Activity >90%; Peroxide<10ppm;

Storage Conditions:

• Store at -20 ⁰C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
• PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

mPEG benzaldehyde

Description:

mPEG benzaldehyde is a monofunctional, site specific PEG derivative with amine reactive aldehyde group. Aldeyde PEG is a common N-terminal amine group pegylation reagent. The reaction between aldehyde group with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups. Aromatic aldehyde PEG shows better stability in aqueous solution.

Physical Properties:

• Off-white/white solid;
• Soluble in water, DMSO, DMF;
• Activity >90%; Peroxide<10ppm;

Storage Conditions:

• Store at -20 ⁰C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
• PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

mPEG benzaldehyde

Description:

mPEG benzaldehyde is a monofunctional, site specific PEG derivative with amine reactive aldehyde group. Aldeyde PEG is a common N-terminal amine group pegylation reagent. The reaction between aldehyde group with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups. Aromatic aldehyde PEG shows better stability in aqueous solution.

Physical Properties:

• Off-white/white solid;
• Soluble in water, DMSO, DMF;
• Activity >90%; Peroxide<10ppm;

Storage Conditions:

• Store at -20 ⁰C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
• PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.