Agarose, S3 High Capacity Acyl-rac Capture Resin, 5 mL
Similar products: Activated thiol Sepharose, activated thiol agarose, thiopropyl Sepharose 6B, thiopropyl activated agarose.
Applications: acyl-RAC protein assays; Site-specific protein immobilization; Protein purification.
High capacity thiol reactive,S3TM acyl-rac capture Resin is one of Nanocs reactive agarose beads that can be used for reversible caputure and release proteins containing free thiol groups, an excellent tool for acyl-rac protein capture and assay use. S3TM thiol capture beads contain reactive pyridyldisulfide bonds that can react easily with free thiol groups under mild conditions. Reaction between thiol and pyridyl disulfide results a reversible dithio bond that can be cleaved by common dithio reducing agents such as DTT or TCEP. Compared to other thiol reactive beads, Nanocs S3TM Beads have exceptionally high thiol reaction capacity; Density of thiol reactive groups on S3 beads is 30-50 times over conventional thiol activated agarose beads. S3 beads can be used in many applications, such as acyl-RAC protein assays; Site-specific protein immobilizations; Protein purification, digestion and analysis. S3 beads are ideal replacements for commonly used activated Thiol-Sepharose 4B or Thiopropyl Sepharose 6B from GE.
- High thiol binding capacity;
- Superior thiol reactivity;
- Reversible disulfide linkage;
- Low non-specific binding;
- Bead Matrix: 6B pegylated agarose beads;
- Bead Size: 50~150 microns;
- Ligand type: Reactive dithiopyridine;
- Ligand density: 800-1000 umol/g beads;
- Reactive to: Free sulfhydryl/thiol groups (cystein, etc.);
- Form: 50% aqueous suspension.
- Store at 4-8 0C.
Acyl-rac Related Reagents:
- DTT (Dithiothreitol)
- Ellman's reagent
- mPEG mal
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