DODMA (1,2-dioleyloxy-3-dimethylaminopropane)

Synonym: 1,2-Dioleyloxy-3-dimethylaminopropane (DODMA)

Description:

DODMA (1,2-dioleyloxy-3-dimethylaminopropane) is an ionizable cationic lipid with a protonatable tertiary amine head group that shows positive charge at low pH. DODMA is highly efficient at encapsulating nucleic acid to form lipoplexes during synthesis by reducing the physiological pH, which readily passes through the cell membrane. These properties make DODMA highly suitable for transfection and drug delivery applications.

Specifications:

• Form: Liquid;
• Color: Colorless to light yellow;
• Molecule Formular: C41H81NO2;
• Molecular weight: 620.09;
• Lipid type: C18 un-saturated, ionizable lipids;
• Applications: Plasmid DNA, siRNA/miRNA and CRISPR/Cas9 delivery.

Storage:

• Store at -20 ⁰C; Protect from light.

DOTAP Methyl Sulfate

Synonym: 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) methyl sulfate

Description:

DOTAP MS (N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate) is a proven reagent for the highly efficient liposomal transfection of DNA, RNA, oligonucleotides, and some other negatively charged large biomolecules. Several other techniques of introducing genetic material into cells may suffer from inadequate reproducibility, cytotoxicity, inconvenience of process, and low transfection efficiency. DOTAP avoids these drawbacks and works by encapsulating the negatively charged DNA within a spontaneously formed liposomal vesicle. This DOTAP/DNA complex can then fuse with the cell membrane and insert DNA directly into the cytoplasm. This mechanism of action ensures transfection occurs efficiently and with little to no cytotoxicity.

Specifications:

• Form: Solid;
• Color: White;
• Molecular formular: C43H83NO8S;
• Molecular weight: 774.21;
• Applications: Mammalian cell transfection with DNA, RNA, and oligonucleotides;

Storage:

• Store at -20 ⁰C.

DODAP (1,2-dioleoyl-3-dimethylammonium-propane)

Description:

DODAP (1,2-dioleoyl-3-dimethylammonium-propane) is an ionizable cationic lipid with low cytotoxicity and high transfection efficiency. DODAP is neutral at physiological pH, but acquires a positive charge inside the endosome due to the protonation of free amines when pH is lower than its pKa (<7). The electrostatic interactions between DODAP and naturally occurring anionic lipids in endosomal membranes trigger the release of nucleic acid. These interactions promote membrane lytic non-bilayer structures to enable the intracellular delivery of nucleic acid. A common application is in nanomedicine as an ionizable lipid component of nanocarriers (lipid-polymer hybrid nanoparticles, LPNs), widely used to encapsulate bioactive molecules, including mRNA, siRNA, and plasmid DNA as a treatment for diseases.

Specifications:

• Form: Liquid;
• Color: Light yellow liquid;
• Formular: C41H77NO4;
• Molecular weight: 648.05;
• Lipid type: C18, un-saturated ionizable lipids;

Applications:

• DNA, SiRNA, mRNA transfection;
• Drug delivery;
• Gene therapy;

Storage:

• Store at -20 ⁰C. Protect from light.

Agarose beads, Cholesterol Conjugated

Synonym: Cholesterol conjugated beads, cholesterol agarose beads

Product Specification:

• Form: Aqueous suspension;
• Color: Colorless;
• Bead type: 4% agarose, crosslinked;
• Coating: Cholesterol;
• Loading ratio: 30-50 umol of Cholesterol/mL beads.

Storage Conditions:

• Store at 4 ⁰C. Do not freeze.

DMG-PEG2000-Thiol

Synonym: PEG2000-DMG-Thiol, DMG-PEG2000-SH, Thiol-DMG-PEG2000, DMG-PEG2000-Sulfhydryl, HS-PEG2000-DMG,  1,2-dimyristoyl-rac-glycero-3-polyethylene glycol 2000 Thiol

Product Specifications:

• Form: Solid
• Color: White
• Lipid type: DMG (1,2-dimyristoyl-rac-glycerol), C14 saturated
• Functional tag: Thiol, sulfhydryl
• Reactive to: Maleimide, gold surface
• PEG type: 2000 PEG, 45 PEG units

Storage:

• Store at -20 ⁰C. Protect from Light.

DMG-PEG2000-Amine

Synonym: PEG2000-DMG-Amine, DMG-PEG2000-NH2, Amino-DMG-PEG2000, DMG-PEG2000-Amino, NH2-PEG2000-DMG,  1,2-dimyristoyl-rac-glycero-3-polyethylene glycol 2000 amine

Product Specifications:

• Form: Solid
• Color: White
• Lipid type: DMG (1,2-dimyristoyl-rac-glycerol), C14 saturated
• Functional tag: Amine, amino (-NH2)
• Reactive to: Succinimidyl ester, carboxylic acid, etc
• PEG type: 2000 PEG, 45 PEG units

Storage:

• Store at -20 ⁰C.

DMG PEG2000 FITC

Synonym: PEG2000-DMG-FITC, DMG-PEG2000-Fluorescein, FITC-DMG-PEG2000, DMG-PEG2000-FITC, FITC-PEG2000-DMG,  1,2-dimyristoyl-rac-glycero-3-polyethylene glycol 2000 FITC

Product Specifications:

• Form: Solid
• Color: Yellow/orange
• Lipid type: DMG (1,2-dimyristoyl-rac-glycerol), C14 saturated
• Functional tag: FITC/fluorescein, fluorescent
• Ex/Em wavelength: 495 nm/515 nm
• PEG type: 2000 PEG, 45 PEG units

Storage:

• Store at -20 ⁰C. Protect from Light.

DMG PEG2000 COOH

Synonym: PEG2000-DMG-Carboxylic acid, DMG-PEG2000-COOH, COOH-DMG-PEG2000, DMG-PEG2000-Acid, Carboxylic-acid-PEG2000-DMG,  1,2-dimyristoyl-rac-glycero-3-polyethylene glycol 2000 acid

Product Specifications:

• Form: Solid
• Color: White
• Lipid type: DMG (1,2-dimyristoyl-rac-glycerol), C14 saturated
• Functional tag: Carboxylic acd, -COOH
• Reactive to: Amine, hydroxyl, etc.
• PEG type: 2000 PEG, 45 PEG units

Storage:

• Store at -20 ⁰C.

DMG PEG2000 Alexa Fluor 647

Synonym: PEG2000-DMG-Alexa fluor 647, DMG-PEG2000-Alexa Fluor 647, AF647-DMG-PEG2000, DMG-PEG2000-Cy5, Cy5-PEG2000-DMG,  1,2-dimyristoyl-rac-glycero-3-polyethylene glycol 2000 Alexa fluor 647

Similar products: Cy5 DMG- PEG2000, SF650 PEG2000-DMG

Product Specifications:

• Form: Solid
• Color: Blue
• Lipid type: DMG (1,2-dimyristoyl-rac-glycerol), C14 saturated
• Functional tag: Alexa Fluor 647, near IR cyanine dye
• Ex/Em wavelength: 650 nm/670 nm
• PEG type: 2000 PEG, 45 PEG units

Storage:

• Store at -20 ⁰C. Protect from Light.

Cholesterol PEG Cy5

Synonym: Cy5 PEG Cholesterol, Chol PEG Cy5

Product Specification:

• Form: Solid;
• Color: Blue;
• Lipid type: Cholestoerl;
• Fluorophore type: Cy5;
• Ex/Em wavelength: 648/670 nm;
• PEG type: Linear PEG;
• Solubility: Soluble in water.

Storage Conditions:

• Store at 4 ⁰C or lower. Protect from light.

DMG PEG2000 Pyrene

Synonym: PEG2000-DMG-Pyrene, DMG-PEG2000-Pyrene, Pyrene-DMG-PEG2000, Pyrene-PEG2000-DMG,  1,2-dimyristoyl-rac-glycero-3-polyethylene glycol 2000 pyrene

Product Specifications:

• Form: Solid
• Color: White
• Lipid type: DMG (1,2-dimyristoyl-rac-glycerol), C14 saturated
• Functional tag: Pyrene, fluorescent
• Ex/Em: 339/384 nm
• PEG type: 2000 PEG, 45 PEG units

Storage:

• Store at -20 ⁰C. Protect from light.

DMG-PEG2000-Cy5

Synonym: PEG2000-DMG-Cy5, DMG-PEG2000-Cy5, Cy5-DMG-PEG2000, DMG-PEG2000-Cy5, Cy5-PEG2000-DMG,  1,2-dimyristoyl-rac-glycero-3-polyethylene glycol 2000 Alexa fluor 647

Similar products: Alexa fluor 647 DMG- PEG2000, SF650 PEG2000-DMG

Product Specifications:

• Form: Solid
• Color: Blue
• Lipid type: DMG (1,2-dimyristoyl-rac-glycerol), C14 saturated
• Functional tag: Cy5, near IR cyanine dye
• Ex/Em wavelength: 650 nm/670 nm
• PEG type: 2000 PEG, 45 PEG units

Storage:

• Store at -20 ⁰C. Protect from Light.

DMG PEG2000 Azide

Synonym: DMG-PEG2000-Azide, DMG-PEG2000-N3, Azide PEG2000 DMG, Azido PEG2000 DMG, 1,2-dimyristoyl-rac-glycero-3-polyethylene glycol 2000

Product Specifications:

• Form: Solid
• Color: White
• Lipid type: DMG (1,2-dimyristoyl-rac-glycerol), C14 saturated
• Functional tag: Azide
• Reactive to: Alkyl/DBCO
• PEG type: 2000 PEG, 45 PEG units

Storage:

• Store at -20 ⁰C. Retest 12 months after receiving.

Cy3 DMG PEG2000

Synonym: PEG2000-DMG-Cy3, DMG-PEG2000-Cy3, Cy3-DMG-PEG2000, DMG-PEG2000-Cy3, Cy3-PEG2000-DMG,  1,2-dimyristoyl-rac-glycero-3-polyethylene glycol 2000 Alexa fluor 555

Similar products: Alexa fluor 555 DMG- PEG2000, SF555 PEG2000-DMG

Product Specifications:

• Form: Solid
• Color: Pink
• Lipid type: DMG (1,2-dimyristoyl-rac-glycerol), C14 saturated
• Functional tag: Cy3, red fluorescent cyanine dye
• Ex/Em wavelength: 550 nm/570 nm
• PEG type: 2000 PEG, 45 PEG units

Storage:

• Store at -20 ⁰C. Protect from Light.

DMG PEG2000 Alkyle

Synonym: DMG-PEG2000-Alkye, Alkye PEG2000 DMG,  1,2-dimyristoyl-rac-glycero-3-polyethylene glycol 2000 alkye

Product Specifications:

• Form: Solid
• Color: White
• Lipid type: DMG (1,2-dimyristoyl-rac-glycerol), C14 saturated
• Functional tag: Alkye
• Reactive to: Azide
• PEG type: 2000 PEG, 45 PEG units

Storage:

• Store at -20 ⁰C. Retest 12 months after receiving.

Cholesterol PEG Cy3

Synonym: Cy3 PEG Cholesterol, Cyanine 3 PEG Cholesterol, Cholesterol PEG Cy3

Similar products: Alexa fluor 555 PEG cholesterol, Cholesterol PEG SF555

Product Specification:

• Form: Solid;
• Color: Pink;
• Lipid type: Cholestoerl;
• Fluorophore type: Cy3, red fluorescent cyanine dye;
• Ex/Em wavelength: 550/570 nm;
• PEG type: Linear PEG;
• Solubility: Soluble in water.

Storage Conditions:

• Store at 4 ⁰C or lower. Protect from light.

DMG PEG2000 DBCO

Synonym: DMG-PEG2000-DBCO, DMG-PEG-DBCO, 2000 Da, DBCO PEG2000 DMG, 1,2-dimyristoyl-rac-glycero-3-polyethylene glycol 2000 DBCO

Product Specifications:

• Form: Solid
• Color: Light yellow
• Lipid type: DMG (1,2-dimyristoyl-rac-glycerol), C14 saturated
• Functional tag: DBCO, Cyclic alkyle
• Reactive to: Azide
• PEG type: 2000 PEG, 45 PEG units

Storage:

• Store at -20 ⁰C. Retest 12 months after receiving.

DMG PEG2000 PDP

Synonym: DMG-PEG2000-PDP, DMG-PEG2000-SPDP, SPDP PEG2000 DMG, PDP PEG2000 DMG, 1,2-dimyristoyl-rac-glycero-3-polyethylene glycol 2000 SPDP

Product Specifications:

• Form: Solid
• Color: White/off-white
• Lipid type: DMG (1,2-dimyristoyl-rac-glycerol), C14 saturated
• Functional tag: SPDP
• Reactive to: Sulfhydryl, form cleavable disulfide bond
• PEG type: 2000 PEG, 45 PEG units

Storage:

• Store at -20 ⁰C. Retest 12 months after receiving.

Biotin PEG400 NHS

Synonym: NHS PEG400 Biotin

Description:

Biotin PEG400 NHS is one of Nanocs pegylated biotinylation reagents. Biotin PEG400 NHS enables simple and efficient biotin labeling of proteins, antibodies or other amine containing molecules or particles. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the biotinylated molecules , thus reducing aggregation of labeled substrates in solution. The PEG spacer arm also gives the biotin a long and flexible structure to minimize steric hindrance for binding to streptavidin or avdins. N-hydroxysulfosuccinimide NHS esters of biotin react efficiently with primary amino groups (-NH2) in alkaline buffer to form a stable amide bond.

Specifications:

• Appearance (color): Off-white;
• Appearance (form): Semi-solid;
• PEG length: PEG400, 8-10 PEG units;
• Reactive group: NHS;
• Reactive to: Primary amine group;
• Solubility: Soluble in DMSO, DMF;
• Application: Biotinylation.

Storage Conditions:

• Store-20 ⁰C. Desiccate. 

SMCC/Maleimidocyclohexane NHS

Name: Succinimidyl 4-(maleimidomethyl)cyclohexane-1-carboxylate, SMCC

Synonym: 4-(N-Maleimidomethyl)cyclohexanecarboxylic acid N-hydroxysuccinimide ester

Description about SMCC:

SMCC, also called Succinimidyl cyclohexane Maleimide, is one of  Nanocs' amine and sulfhydryl reactive crosslinkers. This crossliner is non-cleavable and membrane permeable. It contains an amine reactive NHS ester and a sulfhydryl reactive maleimide group. Compared to other maleimide NHS ester, SMCC has higher stability in aqueous solution because of its cyclohexane bridge. NHS esters react with primary amines at pH 7-9 while the maleimide group reacts with available thiol or sulfhydryl at pH 6.5~7.5 to form stable thioether bonds.

Physical Properties of SMCC:

• Appearance: Off-white/white solid;
• Molecular formula: C16H18N2O6;
• Molecular weight: 334.32;
• Spacer arm length: 8.3 angstrom, 9 atoms
• CAS number: 64987-85-5;
• Purity: >95%
• Soluble in DMF or DMSO;

Storage Conditions of SMCC:

• SMCC should be stored at -20 ⁰C or lower. Desiccate. Avoid frequent thaw and freeze.