Succinimidyl valeric acid PEG, mPEG-SVA
N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol valeric acid (PEG-SVA) is an amino (-NH2) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl valeric acid PEG (mPEG-SVA) offers superior reactivity yet higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.
- Off-white/white solid or viscous liquid depends on molecule weight;
- 10 mg/mL, clear in water, DMSO, Chloroform;
- Store at -20 0C, desiccated. NHS PEG tends to hydrolyze from moisture. Avoid frequent thaw and frozen.
Generally, a 10- to 50-fold molar excess of NHS PEG over the amount of amine-containing material results in sufficient conjugation.
- Pegylation buffer: PBS buffer, pH 7.4 or other amine-free buffer at pH 7-8.5.
- PEG NHS stock solution: 50 mg in 1 mL conjugation buffer.
- Washing solution: Distilled water or any aqueous buffer.
Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add NHS PEG stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of PEG NHS needed for optimal conjugation; Allow mixture agitates at room temperature for 30~60 min at room temperature or 2 hours at 4 0C.Conjugate can be purified either by size exclusion chromatography or dialysis.
*Note: Structure showed here mainly represents the functional groups derived from PEG. Exact linker may not show. Contact us if you have any concern about the linker.