Nanocs SnapLinkTM pegylated succinimidyl cyclohexane Maleimide (SMCC) amine is a heterobifunctional crosslinker that can react with sulfhydryl and amine reactive groups, such as NHS ester. This crosslinker contains a sulfhydryl reactive maleimide group and a primary amine group bridged with PEG arm. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the crosslinked molecules , thus reducing aggregation of labeled proteins in solution. The PEG spacer arm also gives the cyclohexane maleimide PEG amine a long and flexible structure to minimize steric hindrance for its reaction to other molecues. Compared to other maleimide NHS ester, SMCC has higher stability in aqueous solution because of its cyclohexane bridge. Maleimide group reacts with available thiol or sulfhydryl at pH 6.5~7.5 to form stable thioether bonds.
- Appearance: Off-white/white solid or semi-solid;
- Reactivity: Reactive to thiols and activitated carboxylic groups.
- Soluble in DMF or DMSO;
- Store at -20 0C, desiccate. Avoid frequent thaw and freeze.