DBCO-PEG2000-Cy3 Cat. No. PG2-DBS3-2k 5 mg修饰性聚乙二醇



Cat. No. PG2-DBS3-2k
Unit Size 5 mg
Price $385.00

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DBCO (Dibenzocyclooctyne) is a cycloalkyne that can reacts with azides via strain-promoted 1,3-dipolar cycloaddition in aqueous solution, a bioorthogonal reaction also termed Cu-free click reaction.  This reaction has excellent selectivity and biocompatibility, such that the complimentary reagents can form covalent bonds with richly functionalized biological systems. The Cu-free click reaction has been a powerful tool in catalyst-free bioconjugation. DBCO reagents posses fast kinetcs and stability in aqueous buffer and they can be used to label azide-modified biomolecules with high specificity and reactivity. 

Physical Properties:

  • Yellow/pale-yellow solid or semi-solid;
  • Soluble in DMSO or DMF;

Storage Conditions:

  • Store at -20 0C, desiccate. Avoid frequent thaw and frozen.

Copper-free Click Reaction Procedures:

Prepare the azide-containing sample in reaction buffer. Add DBCO conjugate to azide containing sample. Recommendation: Add 1 mole equivalent of limiting reagent to 1.5-3.0 mole equivalents of highest abundance reagent. Incubate the reaction at room temperature for 2-4 hours or 2-12 hours at 4 degree celcius. The reaction is now ready for purification.