Agarose beads, Cholesterol Conjugated

Synonym: Cholesterol conjugated beads, cholesterol agarose beads

Product Specification:

• Form: Aqueous suspension;
• Color: Colorless;
• Bead type: 4% agarose, crosslinked;
• Coating: Cholesterol;
• Loading ratio: 30-50 umol of Cholesterol/mL beads.

Storage Conditions:

• Store at 4 ⁰C. Do not freeze.

DMG PEG2000 Azide

Synonym: DMG-PEG2000-Azide, DMG-PEG2000-N3, Azide PEG2000 DMG, Azido PEG2000 DMG, 1,2-dimyristoyl-rac-glycero-3-polyethylene glycol 2000

Product Specifications:

• Form: Solid
• Color: White
• Lipid type: DMG (1,2-dimyristoyl-rac-glycerol), C14 saturated
• Functional tag: Azide
• Reactive to: Alkyl/DBCO
• PEG type: 2000 PEG, 45 PEG units

Storage:

• Store at -20 ⁰C. Retest 12 months after receiving.

Vitamin D3 PEG Biotin

Synonym: Cholecalciferol PEG Biotin, Biotin PEG Vitamin D3, Calciol PEG Biotin, Vitamin D PEG2000 Biotin

Product Information:

• Appearance: White/off-white powder
• Solubility: Soluble in water, DMSO
• Binding target: Avidin, streptavidin or neutravidin
• Unit size: 5 mg

Storage:

• Store at -20 ⁰C. Protect from light.

Graphene oxide, aqueous solution

Graphene Oxide Description:

Nanocs provides single layer graphene (SLG) and ,strong>graphene oxide (SLGO) products for your research use. These graphene products have been processed with multi step chemical and physical processes to ensure high quality.

Nanocs also provides various surface modified graphene and graphene oxide products with a wide range of specifications to suit different applications.

Product Specifications:

• Composition: Single layer graphene oxide in aqueous solution, 2~5 mg/mL graphene oxide;
• Flake Size: 0.5~5 microns;
• Thickness: 1.1 nm. Single atomic layer over 80%;
• Purity: Carbon contents around 75%, oxygen contents 35%;
• Color: Pale yellow to dark brown.

Storage Conditions:

• Store at 4 ⁰C.

Graphene oxide, biotin labeled

Description:

Nanocs provides various single layer graphene and graphene oxide products for your research use. These graphene products have been processed with multi step chemical and physical processes to ensure high quality.

Nanocs also provides various surface modified graphene and graphene oxide products with a wide range of specifications to suit different applications.

Product Specifications:

• Composition: Single layer graphene oxide in aqueous solution, 2~5 mg/mL graphene oxide;
• Flake Size: 0.5~5 microns;
• Thickness: 1.1 nm. Single atomic layer over 80%;
• Purity: Carbon contents around 75%, oxygen contents 35%;
• Color: Pale yellow to dark brown.

Storage Conditions:

• Store at 4 ⁰C.

5(6)-Carboxyfluorescein

Azido succinimidyl acetic acid NHS

DBCO-PEG4-NHS

Description:

DBCO (Dibenzocyclooctyne) is a cycloalkyne that can reacts with azides via strain-promoted 1,3-dipolar cycloaddition in aqueous solution, a bioorthogonal reaction also termed Cu-free click reaction.  This reaction has excellent selectivity and biocompatibility, such that the complimentary reagents can form covalent bonds with richly functionalized biological systems. The Cu-free click reaction has been a powerful tool in catalyst-free bioconjugation. DBCO reagents posses fast kinetcs and stability in aqueous buffer and they can be used to label azide-modified biomolecules with high specificity and reactivity. 

Physical Properties:

• Yellow/pale-yellow solid or viscous liquid;
• Soluble in DMSO or DMF;

Storage Conditions:

• Store at -20 ⁰C, desiccate. NHS tends to hydrolyze from moisture. Avoid frequent thaw and frozen.

Reaction Procedures:

NHS esters are moisture senstivie. To avoid moisture condensation onto the product always let vial come to room temperature before opening; be exposure to limit exposure to moisture and restore under atmosphere. The NHS ester moiety readily hydrolyzes and becomes non-reactive; therefore, prepare stock solutions immediately before use. Stock solutions in anhydrous solvents can be kept for several days (freeze when not in use). Hydrolysis of the NHS ester is a competing reaction. Conjugation with primary amines of proteins/peptides (i.e., acylation) is favored at near neutral pH (6-9) and with concentrated protein solutions. For conjugation, use non-amine containing buffers at pH 7-9 such as PBS (20 mM sodium phosphate, 150 mM sodium chloride, pH 7.4); 20 mM HEPES; 100 mM carbonate/biocarbonate; or 50 mM borate buffer. Do not use buffers that contain primary amines, (e.g., Tris, glycine). Avoid buffers that contain azides, which can react with DBCO. Dissolve DBCO water miscible organic solvent such as DMSO or DMF before diluting in final reaction buffer. DBCO-NHS ester is not soluble in aqueous buffers.

Materials Required:

• Conjugation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-free buffer at pH 7-8.5.
• Water miscible solvents: DMSO or DMF.
• Quenching buffer: 1 M Tris.HCl, pH 8.0.

Reaction Steps:

Prepare proteins in PBS. Immediately before use, prepare 10 mM of the DBCO-NHS reagent in DMSO or DMF. Add the NHS reagent to the protein sample at a final: mM. If For samples <5 mg/ml, use a 20 to 50 fold molar excess. Incubate the reaction at room temperature for 30 minutes or on ice for 2 hours. Stop the reaction by adding Quenching Buffer 100 mM Tris.or on ice for 15 minutes. Remove non reagentby dialysis or desalting.

Copper-free Click Reaction:

Prepare the azide-containing sample in reaction buffer. Add DBCO conjugate to azide containing sample. Recommendation: Add 1 mole equivalent of limiting reagent to 1.5-3.0 mole equivalents of highest abundance reagent. Incubate the reaction at room temperature for 2-4 hours or 2-12 hours at 4 degree celcius. The reaction is now ready for purification.

DBCO-PEG2000-maleimide

Description:

DBCO (Dibenzocyclooctyne) is a cycloalkyne that can reacts with azides via strain-promoted 1,3-dipolar cycloaddition in aqueous solution, a bioorthogonal reaction also termed Cu-free click reaction.  This reaction has excellent selectivity and biocompatibility, such that the complimentary reagents can form covalent bonds with richly functionalized biological systems. The Cu-free click reaction has been a powerful tool in catalyst-free bioconjugation. DBCO reagents posses fast kinetcs and stability in aqueous buffer and they can be used to label azide-modified biomolecules with high specificity and reactivity. 

Physical Properties:

• Yellow/pale-yellow solid or semi-solid;
• Soluble in DMSO or DMF;

Storage Conditions:

• Store at -20 ⁰C, desiccate. Avoid frequent thaw and frozen.

Copper-free Click Reaction Procedures:

Prepare the azide-containing sample in reaction buffer. Add DBCO conjugate to azide containing sample. Recommendation: Add 1 mole equivalent of limiting reagent to 1.5-3.0 mole equivalents of highest abundance reagent. Incubate the reaction at room temperature for 2-4 hours or 2-12 hours at 4 degree celcius. The reaction is now ready for purification.

DBCO-PEG2000-Cy3

Description:

DBCO (Dibenzocyclooctyne) is a cycloalkyne that can reacts with azides via strain-promoted 1,3-dipolar cycloaddition in aqueous solution, a bioorthogonal reaction also termed Cu-free click reaction.  This reaction has excellent selectivity and biocompatibility, such that the complimentary reagents can form covalent bonds with richly functionalized biological systems. The Cu-free click reaction has been a powerful tool in catalyst-free bioconjugation. DBCO reagents posses fast kinetcs and stability in aqueous buffer and they can be used to label azide-modified biomolecules with high specificity and reactivity. 

Physical Properties:

• Yellow/pale-yellow solid or semi-solid;
• Soluble in DMSO or DMF;

Storage Conditions:

• Store at -20 ⁰C, desiccate. Avoid frequent thaw and frozen.

Copper-free Click Reaction Procedures:

Prepare the azide-containing sample in reaction buffer. Add DBCO conjugate to azide containing sample. Recommendation: Add 1 mole equivalent of limiting reagent to 1.5-3.0 mole equivalents of highest abundance reagent. Incubate the reaction at room temperature for 2-4 hours or 2-12 hours at 4 degree celcius. The reaction is now ready for purification.

DBCO PEG2000 Folic acid

Description:

DBCO (Dibenzocyclooctyne) is a cycloalkyne that can reacts with azides via strain-promoted 1,3-dipolar cycloaddition in aqueous solution, a bioorthogonal reaction also termed Cu-free click reaction.  This reaction has excellent selectivity and biocompatibility, such that the complimentary reagents can form covalent bonds with richly functionalized biological systems. The Cu-free click reaction has been a powerful tool in catalyst-free bioconjugation. DBCO reagents posses fast kinetcs and stability in aqueous buffer and they can be used to label azide-modified biomolecules with high specificity and reactivity. 

Physical Properties:

• Yellow/pale-yellow solid or semi-solid;
• Soluble in DMSO or DMF;

Storage Conditions:

• Store at -20 ⁰C, desiccate. Avoid frequent thaw and frozen.

Copper-free Click Reaction Procedures:

Prepare the azide-containing sample in reaction buffer. Add DBCO conjugate to azide containing sample. Recommendation: Add 1 mole equivalent of limiting reagent to 1.5-3.0 mole equivalents of highest abundance reagent. Incubate the reaction at room temperature for 2-4 hours or 2-12 hours at 4 degree celcius. The reaction is now ready for purification.

DBCO PEG2000 Glucose

Description:

DBCO (Dibenzocyclooctyne) is a cycloalkyne that can reacts with azides via strain-promoted 1,3-dipolar cycloaddition in aqueous solution, a bioorthogonal reaction also termed Cu-free click reaction.  This reaction has excellent selectivity and biocompatibility, such that the complimentary reagents can form covalent bonds with richly functionalized biological systems. The Cu-free click reaction has been a powerful tool in catalyst-free bioconjugation. DBCO reagents posses fast kinetcs and stability in aqueous buffer and they can be used to label azide-modified biomolecules with high specificity and reactivity. 

Physical Properties:

• Yellow/pale-yellow solid or semi-solid;
• Soluble in DMSO or DMF;

Storage Conditions:

• Store at -20 ⁰C, desiccate. Avoid frequent thaw and frozen.

Copper-free Click Reaction Procedures:

Prepare the azide-containing sample in reaction buffer. Add DBCO conjugate to azide containing sample. Recommendation: Add 1 mole equivalent of limiting reagent to 1.5-3.0 mole equivalents of highest abundance reagent. Incubate the reaction at room temperature for 2-4 hours or 2-12 hours at 4 degree celcius. The reaction is now ready for purification.

Maleimidoacetic acid NHS

Description:

Nanocs maleidoacetic acid NHS (Maleimide-C2-NHS) is an amine and sulfhydryl crosslinker with a short C2 space arm between maleimide and NHS. This crossliker is non-cleavable and membrane permeable. It contains an amine reactive NHS ester and a sulfhydryl reactive maleimide group. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. The maleimide group reacts with available –SH groups from naturally occurring or mutagenically derived cysteine residues. Maleimide groups react with sulfhydryl groups at pH 6.5~7.5 at room temperature to form stable thioether bonds.

Physical Properties:

• Appearance: Off-white/white solid;
• Purity: >95%
• Solubility: Soluble in DMF or DMSO; Not soluble in water;

Storage Conditions:

• Store at -20 ⁰C, Desiccate.

MBS, Maleimidobenzoyl NHS

Description:

Nanocs m-Maleimidobenzyol N-hydroxysccinimide, also called MBS, is an aromatic, amine and sulfhydryl crosslinker with a short space arm between maleimide and NHS. This crossliker is non-cleavable and membrane permeable. It contains an amine reactive NHS ester and a sulfhydryl reactive maleimide group. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. The maleimide group reacts with available –SH groups from naturally occurring or mutagenically derived cysteine residues. Maleimide groups react with sulfhydryl groups at pH 6.5~7.5 at room temperature to form stable thioether bonds.

Physical Properties:

• Appearance: Off-white/white solid;
• Purity: >95%
• Solubility: Soluble in DMF or DMSO; Not soluble in water;

Storage Conditions:

• Store at -20 ⁰C. Desiccate. Avoid frequent thaw and freeze.

Maleimide-PEG23-NH2

Description:

Nanocs SnapLink^TM pegylated succinimidyl cyclohexane Maleimide (SMCC) amine is a heterobifunctional crosslinker that can react with sulfhydryl and amien reactive groups, such as NHS ester. This crosslinker contains a sulfhydryl reactive maleimide group and a primary amine group bridged with PEG arm. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the crosslinked molecules , thus reducing aggregation of labeled proteins in solution. The PEG spacer arm also gives the cyclohexane maleimide PEG amine a long and flexible structure to minimize steric hindrance for its reaction to other molecues. Compared to other maleimide NHS ester, SMCC has higher stability in aqueous solution because of its cyclohexane bridge. Maleimide group reacts with available thiol or sulfhydryl at pH 6.5~7.5 to form stable thioether bonds.

Physical Properties:

• Appearance: Off-white/white solid or semi-solid;
• Reactivity: Reactive to sulfhydryl and carboxylic groups;
• Soluble in DMF or DMSO;

Storage Conditions:

• Store at -20 ⁰C, desiccate. Avoid frequent thaw and freeze.

SMCC-PEG45-NH2

Description:

Nanocs SnapLink^TM pegylated succinimidyl cyclohexane Maleimide (SMCC) amine is a heterobifunctional crosslinker that can react with sulfhydryl and amine reactive groups, such as NHS ester. This crosslinker contains a sulfhydryl reactive maleimide group and a primary amine group bridged with PEG arm. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the crosslinked molecules , thus reducing aggregation of labeled proteins in solution. The PEG spacer arm also gives the cyclohexane maleimide PEG amine a long and flexible structure to minimize steric hindrance for its reaction to other molecues. Compared to other maleimide NHS ester, SMCC has higher stability in aqueous solution because of its cyclohexane bridge. Maleimide group reacts with available thiol or sulfhydryl at pH 6.5~7.5 to form stable thioether bonds.

Physical Properties:

• Appearance: Off-white/white solid or semi-solid;
• Reactivity: Reactive to thiols and activitated carboxylic groups.
• Soluble in DMF or DMSO;

Storage Conditions:

• Store at -20 ⁰C, desiccate. Avoid frequent thaw and freeze.

ITO Coated Glass Slides, 25x75x1.1 mm

ITO (Inidum Tin Oxide) and FTO (fluorine doped tin oxide) Coated Glass Slides

ITO (Indium Tin Oxide) and FTO coated glass slides provided from Nanocs were made by coating ITO or FTO to one side of soda lime glass. These ITO and/or FTO coated glass, also called transparent conductive glass (TCO), come with different surface resistivity from 5 ohm/sq to 100 ohm/sq, with thickness from 0.5 mm to 2.2 mm. ITO and FTO coated glass has been used in LCD/OLED devices and many other devices requiring high electrical conductivity and good optical transmission. Our ITO/FTO coated glass has excellent surface roughness around +-5 nm. Thickness of our ITO or FTO coating ranges from 50 nm to 700 nm depends on their surface resistivity. Optical transmission of these ITO coated glass is over 85% in the visible range.

Nanocs ITO/FTO Glass Specifications:

• Surface resistivity: 5~100 ohm/sq, see individual product label;
• Glass thickness: 0.55 mm, 1.1 mm or 2.2 mm;
• ITO thickness: 30~600 nm depends on ITO surface resistivity:
• Optical transmission: 83% to 90%;
• ITO glass size: 1 cm to 10 cm, customer size available upon request.

ITO Coated Glass Slides, 25x75x1.1 mm

ITO (Inidum Tin Oxide) and FTO (fluorine doped tin oxide) Coated Glass Slides

ITO (Indium Tin Oxide) and FTO coated glass slides provided from Nanocs were made by coating ITO or FTO to one side of soda lime glass. These ITO and/or FTO coated glass, also called transparent conductive glass (TCO), come with different surface resistivity from 5 ohm/sq to 100 ohm/sq, with thickness from 0.5 mm to 2.2 mm. ITO and FTO coated glass has been used in LCD/OLED devices and many other devices requiring high electrical conductivity and good optical transmission. Our ITO/FTO coated glass has excellent surface roughness around +-5 nm. Thickness of our ITO or FTO coating ranges from 50 nm to 700 nm depends on their surface resistivity. Optical transmission of these ITO coated glass is over 85% in the visible range.

Nanocs ITO/FTO Glass Specifications:

• Surface resistivity: 5~100 ohm/sq, see individual product label;
• Glass thickness: 0.55 mm, 1.1 mm or 2.2 mm;
• ITO thickness: 30~600 nm depends on ITO surface resistivity:
• Optical transmission: 83% to 90%;
• ITO glass size: 1 cm to 10 cm, customer size available upon request.

ITO Coated Glass Slides, 25x75x0.55 mm

ITO (Inidum Tin Oxide) and FTO (fluorine doped tin oxide) Coated Glass Slides

ITO (Indium Tin Oxide) and FTO coated glass slides provided from Nanocs were made by coating ITO or FTO to one side of soda lime glass. These ITO and/or FTO coated glass, also called transparent conductive glass (TCO), come with different surface resistivity from 5 ohm/sq to 100 ohm/sq, with thickness from 0.5 mm to 2.2 mm. ITO and FTO coated glass has been used in LCD/OLED devices and many other devices requiring high electrical conductivity and good optical transmission. Our ITO/FTO coated glass has excellent surface roughness around +-5 nm. Thickness of our ITO or FTO coating ranges from 50 nm to 700 nm depends on their surface resistivity. Optical transmission of these ITO coated glass is over 85% in the visible range.

Nanocs ITO/FTO Glass Specifications:

• Surface resistivity: 5~100 ohm/sq, see individual product label;
• Glass thickness: 0.55 mm, 1.1 mm or 2.2 mm;
• ITO thickness: 30~600 nm depends on ITO surface resistivity:
• Optical transmission: 83% to 90%;
• ITO glass size: 1 cm to 10 cm, customer size available upon request.

ITO Coated Coverslips, 18×18 mm

ITO (Inidum Tin Oxide) Coated Coverslips

ITO (Indium Tin Oxide) coated coverslips provided from Nanocs were made by coating ITO to one side of glass coveslips. These ITO coated glass coverslips come with different surface resistivity from 5 ohm/sq to 100 ohm/sq, with thickness around 0.15~0.17mm. ITO coverslips has been used in LCD/OLED devices and many other devices requiring high electrical conductivity and good optical transmission. Our ITO coated glass coverslips have excellent surface roughness around +-5 nm. Thickness of our ITO coating ranges from 50 nm to 700 nm depends on their surface resistivity. Optical transmission of these ITO coated coverslips is over 85% in the visible range.

Nanocs ITO Coated Coverslip Specifications:

• Surface resistivity: 5~100 ohm/sq, see individual product label;
• Coverslip thickness: 0.15~0.17 mm;
• ITO thickness: 30~600 nm depends on ITO surface resistivity:
• Optical transmission: 83% to 90%;
• ITO coverslip size: 18 mm x 18 mm, 22 mm x 22 mm, 22 mm x 40 mm