DBCO PEG NHS Cat. No. PG2-DBNS-3k 3400 Da 20 mg修饰性聚乙二醇

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DBCO PEG NHS

Cat. No. PG2-DBNS-3k DBCO PEG NHS           Cat. No. PG2-DBNS-3k     3400 Da    20 mg
Specification 3400 Da
Unit Size 20 mg
Price $385.00


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Description:

Dibenzocycolctyne (DBCO) PEG NHS derivatives can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. NHS group, on the other hand, can react with primary amine group at alkaline pH to form a stable amide bond. NHS funtionalized DBCO PEG thus can be easily attached to proteins, antibodies, peptides or small molecules to derive DBCO reactive moiety.

Physical Properties:

  • Light yellow solid;
  • Soluble in water, DMSO, etc.;

Copper-free Click reaction procedures:

  • Prepare the azide-containing reagents in reaction buffer.
  • Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
  • Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
  • Purify conjugates either by dialysis or size-exclusion chromatograph.

Storage Conditions:

  • Store at -20 0C, desiccate. Protect from light. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet