DBCO PEG NHS
Dibenzocycolctyne (DBCO) PEG NHS derivatives can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. NHS group, on the other hand, can react with primary amine group at alkaline pH to form a stable amide bond. NHS funtionalized DBCO PEG thus can be easily attached to proteins, antibodies, peptides or small molecules to derive DBCO reactive moiety.
- Light yellow solid;
- Soluble in water, DMSO, etc.;
Copper-free Click reaction procedures:
- Prepare the azide-containing reagents in reaction buffer.
- Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
- Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
- Purify conjugates either by dialysis or size-exclusion chromatograph.
- Store at -20 0C, desiccate. Protect from light. Avoid frequent thaw and freeze.