Low molecular weight hylauronic acid (LMWHA), Cy7 labeled

Description:

Nanocs provides a variety of chemically functionalized and bioconjugated low molecular weight hylauronic acid (LMWHA) with different functionality. LMWHA has better solubility and permeability. These functional Hylauronic acid products were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

• 20~40 functional groups on each hylauronic acid molecule.
• Hylauronci acid conjugates provided either in water solution and dried powder;

Storage Conditions:

• Store4 ⁰C, dessiccated Protect from light.

Low molecular weight hylauronic acid (LMWHA), Biotin labeled

Description:

Nanocs provides a variety of chemically functionalized and bioconjugated low molecular weight hylauronic acid (LMWHA) with different functionality. LMWHA has better solubility and permeability. These functional Hylauronic acid products were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

• 20~40 functional groups on each hylauronic acid molecule.
• Hylauronci acid conjugates provided either in water solution and dried powder;

Storage Conditions:

• Store4 ⁰C, dessiccated Protect from light.

Chitosan, fluorescein labeled, 180 kDa

Description:

Nanocs provides a variety of chemically functionalized and bioconjugated chitosan polysaccharide with different functionality. These functional chitosan products were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

• 10~50 functional groups on each chitosan molecule.
• Chitosan conjugates provided either in water solution and dried powder;

Storage Conditions:

• Store4 ⁰C, dessiccated Protect from light.

Protein A, HRP labeled

Description:

HRP labeled Protein A is one of Nanocs enzyme protein A conjugates. Nanocs provides a variety of chemically functionalized and bioconjugated protein A with different functionality. These functional protein A products were purified by size exclusion chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

• 2~7 functional groups on each protein A molecule.
• Protein A and it conjugates provided either in water solution or dried powder;

Storage Conditions:

• Store at 4 ⁰C.

Protein A, maleimide function

Description:

Maleimide modified Protein A is one of Nanocs’ reactive protein A conjugates that can be used to react with sulfhydryl groups from proteins, antibodies or peptides. Nanocs provides a variety of chemically functionalized and bioconjugated protein A with different functionality. These functional protein A products were purified by size exclusion chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

• 2~7 functional groups on each protein A molecule.
• Protein A maleimide conjugate is provied in dried powder;

Storage Conditions:

• Store at -20 ⁰C. Desiccate.

Catalase, PEG labeled

Description:

PEG labeled catalase is one of Nanocs’ pegylated catalase conjugates. Catalase is a common enzyme found in nearly all living organisms exposed to oxygen. It catalyzes the decomposition of hydrogen peroxide to water and oxygen. Catalase is a important enzyme in protetecting the cell from oxidative damage by reactive oxygen species. PEG modified catalase shows better stability with good reactivity. Nanocs provides a variety of chemically functionalized and bioconjugated catalase with different functionality. These functionalized catalase conjugates were purified by size exclusion chromatography to ensure adequate applications both in-vitro and in-vivo.

Specifications:

• 20~50 PEG5000 on each catalase molecule.
• Catalase conjugates provided either in water solution or dried powder;

Storage Conditions:

• Store at 4 ⁰C.

Human serum albumin, HSA, maleimide function

Description:

Nanocs provides a variety of chemically functionalized and bioconjugated Human serum albumin (HSA) with different functionality. These functional HSA products were purified by size exclusion chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

• 2~7 functional groups on each HSA molecule.
• HSA conjugates provided either in water solution or dried powder;

Storage Conditions:

• Store at 4 ⁰C. Protect from light.

DNP-BSA, BSA DNP Conjugate

DNP-BSA, 2,4-Dinitrophenyl Albumin (Bovine) conjugate:

DNP-BSA is one of Nanocs 2,4-dinitrophenyl (DNP) albumin (bovin, BSA) conjugate that can be used for immunoassay development. DNP is a frequently used heptan that has strong absorption around 360 nm. Nanocs provides a number of DNP albumin conjugates that can be used for different applications. These DNP conjugates were purified by size exclusion chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

• Form: Powder or buffered solution
• Color: Yellow
• DNP ratio: >10 DNP per BSA
• Source: Synthetic
• Absorption: 360 nm

Storage Conditions:

• Store at <4 ⁰C. Protect from light.

DNP-BSA, BSA DNP Conjugate

DNP-BSA, 2,4-Dinitrophenyl Albumin (Bovine) conjugate:

DNP-BSA is one of Nanocs 2,4-dinitrophenyl (DNP) albumin (bovin, BSA) conjugate that can be used for immunoassay development. DNP is a frequently used heptan that has strong absorption around 360 nm. Nanocs provides a number of DNP albumin conjugates that can be used for different applications. These DNP conjugates were purified by size exclusion chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

• Form: Powder or buffered solution
• Color: Yellow
• DNP ratio: >10 DNP per BSA
• Source: Synthetic
• Absorption: 360 nm

Storage Conditions:

• Store at <4 ⁰C. Protect from light.

Ovalbumin DNP (DNP-OVA) conjugate

DNP-OVA, DNP Ovalbumin Conjugate:

DNP-OVA is one of Nanocs 2,4-dinitrophenyl (DNP) albumin (Ovalbumin from Egg white, OVA) conjugates that can be used for immunoassay development. DNP is a frequently used heptan that has strong absorption around 360 nm. Nanocs provides a number of DNP conjugates that can be used for different applications. These DNP conjugates were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

• Form: Powder
• Color: Yellow
• DNP ratio: >30 DNP per OVA
• Source: Synthetic
• Absorption: 360 nm

Storage Conditions:

• Store at <4 ⁰C. Protect from light.

Ovalbumin DNP (DNP-OVA) conjugate

DNP-OVA, DNP Ovalbumin Conjugate:

DNP-OVA is one of Nanocs 2,4-dinitrophenyl (DNP) albumin (Ovalbumin from Egg white, OVA) conjugate that can be used for immunoassay development. DNP is a frequently used heptan that has strong absorption around 360 nm. Nanocs provides a number of DNP conjugates that can be used for different applications. These DNP conjugates were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

• Form: Powder
• Color: Yellow
• DNP ratio: >30 DNP per OVA
• Source: Synthetic
• Absorption: 360 nm

Storage Conditions:

• Store at <4 ⁰C. Protect from light.

Succinimidyl PEG NHS, mPEG-NHS(SC)

Description:

N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH₂) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.

Physical Properties:

• Off-white/white solid or viscous liquid depends on molecule weight;
• Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

• Store at -20 ⁰C, desiccate. NHS PEG tends to hydrolyze from moisture. Avoid frequent thaw and frozen.

Reaction Procedures:

Generally, a 10 to 50-fold molar excess of NHS PEG over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Conjugation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-free buffer at pH 7-8.5.
• PEG NHS stock solution: 100 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in conjugation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add NHS PEG stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of PEG NHS needed for optimal conjugation; Allow mixture agitates at room temperature for 30~60 min at room temperature or 2 hours at 4 ⁰C. Conjugates can be purified either by size exclusion chromatography or dialysis.

Succinimidyl PEG NHS, mPEG-NHS(SC)

Description:

N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH₂) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.

Physical Properties:

• Off-white/white solid or viscous liquid depends on molecule weight;
• Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

• Store at -20 ⁰C, desiccate. NHS PEG tends to hydrolyze from moisture. Avoid frequent thaw and frozen.

Reaction Procedures:

Generally, a 10 to 50-fold molar excess of NHS PEG over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Conjugation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-free buffer at pH 7-8.5.
• PEG NHS stock solution: 100 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in conjugation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add NHS PEG stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of PEG NHS needed for optimal conjugation; Allow mixture agitates at room temperature for 30~60 min at room temperature or 2 hours at 4 ⁰C. Conjugates can be purified either by size exclusion chromatography or dialysis.

Succinimidyl acid PEG, mPEG-NHS

Description:

N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH₂) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.

Physical Properties:

• Off-white/white solid or viscous liquid depends on molecule weight;
• Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

• Store at -20 ⁰C, desiccate. NHS PEG tends to hydrolyze from moisture. Avoid frequent thaw and frozen.

Reaction Procedures:

Generally, a 10 to 50-fold molar excess of NHS PEG over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Conjugation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-free buffer at pH 7-8.5.
• PEG NHS stock solution: 100 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in conjugation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add NHS PEG stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of PEG NHS needed for optimal conjugation; Allow mixture agitates at room temperature for 30~60 min at room temperature or 2 hours at 4 ⁰C. Conjugates can be purified either by size exclusion chromatography or dialysis.

Succinimidyl acid PEG, mPEG-NHS

Description:

N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH₂) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.

Physical Properties:

• Off-white/white solid or viscous liquid depends on molecule weight;
• Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

• Store at -20 ⁰C, desiccate. NHS PEG tends to hydrolyze from moisture. Avoid frequent thaw and frozen.

Reaction Procedures:

Generally, a 10 to 50-fold molar excess of NHS PEG over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Conjugation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-free buffer at pH 7-8.5.
• PEG NHS stock solution: 100 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in conjugation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add NHS PEG stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of PEG NHS needed for optimal conjugation; Allow mixture agitates at room temperature for 30~60 min at room temperature or 2 hours at 4 ⁰C. Conjugates can be purified either by size exclusion chromatography or dialysis.

NHS-PEG-NHS

Description:

N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH₂) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.

Physical Properties:

• Off-white/white solid or viscous liquid depends on molecule weight;
• Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

• Store at -20 ⁰C, desiccate. NHS PEG tends to hydrolyze from moisture. Avoid frequent thaw and frozen.

Reaction Procedures:

Generally, a 10 to 50-fold molar excess of NHS PEG over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Conjugation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-free buffer at pH 7-8.5.
• PEG NHS stock solution: 100 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in conjugation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add NHS PEG stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of PEG NHS needed for optimal conjugation; Allow mixture agitates at room temperature for 30~60 min at room temperature or 2 hours at 4 ⁰C. Conjugates can be purified either by size exclusion chromatography or dialysis.

PEG Aldehyde, mPEG-CH2CHO

PEG Aldehyde Description:

Aldeyde PEG is an amine reactive PEG derivative that can be used to modify biomolecules via available amine groups. The reaction between aldehyde group with ε-amine of lysine residues and the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with amine groups at a pH of from 5.5 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups. Aldehyde PEG is a commonly used PEG reagent for n-terminus pegylation in proteins or peptides.

Physical Properties:

• Off-white/white solid or viscous liquid depends on molecule weight;
• Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

• Store at -20 ⁰C

Reaction Procedures:

Generally, a 10- to 50-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Pegylation buffer: Amine-free aqueous buffer, pH 6~8.5.
• PEG aldehyde stock solution: 100 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Aldehyde PEG Aldehyde,CHO-PEG-CHO

PEG Aldehyde Description:

Aldeyde PEG is an amine reactive PEG derivative that can be used to modify biomolecules via available amine groups. The reaction between aldehyde group with ε-amine of lysine residues and the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with amine groups at a pH of from 5.5 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups. Aldehyde PEG is a commonly used PEG reagent for n-terminus pegylation in proteins or peptides.

Physical Properties:

• Off-white/white solid or viscous liquid depends on molecule weight;
• Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

• Store at -20 ⁰C

Reaction Procedures:

Generally, a 10- to 50-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Pegylation buffer: Amine-free aqueous buffer, pH 6~8.5.
• PEG aldehyde stock solution: 100 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 ⁰C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Nitrophenyl PEG, mPEG-NPC

Description:

Nitrophenyl functional PEG, NPC PEG is an amino (-NH₂) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with available amino groups either on their lysine residue or N-terminus. NPC PEGs react readily with amine groups at a pH of from 6.5 to 9.5.

Physical Properties:

• Off-white/white solid or viscous liquid depends on molecule weight;
• Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

• Store at -20 ⁰C. Dessicated.

Nitrophenyl PEG, mPEG-NPC

Description:

Nitrophenyl functional PEG, NPC PEG is an amino (-NH₂) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with available amino groups either on their lysine residue or N-terminus. NPC PEGs react readily with amine groups at a pH of from 6.5 to 9.5.

Physical Properties:

• Off-white/white solid or viscous liquid depends on molecule weight;
• Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

• Store at -20 ⁰C. Dessicated.