DSPE PEG Aldehyde
Synonym: 1,2-distearoyl-sn-glycero-3-phosphoethanolamine PEG aldehyde, DSPE-PEG-CHO, Aldehyde PEG DSPE
DSPE PEG aldehyde (DSPE-PEG-CHO) is one of Nanocs’ reactive phospholipid PEG derivatives that can react with N-terminus amine groups. DSPE (1,2-distearoyl-sn-glycero-3-phosphoethanolamine) is an 18 carbon phospholipid that is highly hydrophobic. PEG backbone, on the other hand, offers good hydrophilicity. Aldehyde PEG DSPE is a reactive lipid PEG with good reactivity towards amine, oxyamine or hydrazide groups. Aldehyde reacts with N-terminus amine groups to form an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. Aldehyde PEG DSPE allows conjugation of DSPE PEG to amine or hydrazide group at acidic conditions with good selectivity. Higher pH will result in multiple pegylation with amine groups.
- Form: Solid;
- Color: White;
- Reactive group: Aldehyde (-CHO)
- Reactive to: N-terminus amine
- Purity: >90%
- Solubility: Soluble in warm water;
- Store at -20 0C
Generally, a 5- to 10-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.
- Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
- PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
- Washing solution: Distilled water or any aqueous buffer.
Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.