Rabbit IgG, Gold Conjugate

Rabbit IgG, Gold Conjugate

Human IgG, Gold Conjugate

Low molecular weight hylauronic acid (LMWHA), Cy3 labeled

Description:

Nanocs provides a variety of chemically functionalized and bioconjugated low molecular weight hylauronic acid (LMWHA) with different functionality. LMWHA has better solubility and permeability. These functional Hylauronic acid products were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

• 20~40 functional groups on each hylauronic acid molecule.
• Hylauronci acid conjugates provided either in water solution and dried powder;

Storage Conditions:

• Store4 ⁰C, dessiccated Protect from light.

Chitosan, Cy5 labeled, 120 kDa

Description:

Nanocs provides a variety of chemically functionalized and bioconjugated chitosan polysaccharide with different functionality. These functional chitosan products were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

• 10~50 functional groups on each chitosan molecule.
• Chitosan conjugates provided either in water solution and dried powder;

Storage Conditions:

• Store4 ⁰C, dessiccated Protect from light.

Chitosan, Cy5.5 labeled, 120 kDa

Description:

Nanocs provides a variety of chemically functionalized and bioconjugated chitosan polysaccharide with different functionality. These functional chitosan products were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

• 10~50 functional groups on each chitosan molecule.
• Chitosan conjugates provided either in water solution and dried powder;

Storage Conditions:

• Store4 ⁰C, dessiccated Protect from light.

Protein A, Rhodamine labeled

Description:

Rhodamine B labeled Protein A is one of the Nanocs’ premier fluorescent Protein A conjugates. Protein A is a 42 kDa surface protein originally found in the cell wall of Stapylococcus aureus. Protein A has strong binding affinity towards immunoglobulins and it has been used for antibody identification, purification and many other biochemical research uses. Rhodamine protein A is a red fluorescent protein A conjugate that has excitation/emission wavelength at 544 nm/576 nm. All our protein A conjugates were purified by size exclusion chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

• 2~7 Rhodamine B dyes on each protein A molecule.
• Protein A and it conjugates provided either in water solution or dried powder;

Storage Conditions:

• Store at 4 ⁰C. Protect from light.

Protein A, Cy5 labeled

Description:

Cy5 labeled Protein A is one of the Nanocs’ premier fluorescent Protein A conjugates. Protein A is a 42 kDa surface protein originally found in the cell wall of Stapylococcus aureus. Protein A has strong binding affinity towards immunoglobulins and it has been used for antibody identification, purification and many other biochemical research uses. Cy5 protein A is a near IR fluorescent protein A conjugate that has excitation/emission wavelength at 650 nm/670 nm. All our protein A conjugates were purified by size exclusion chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

• 2~7 Cy5 dyes on each protein A molecule.
• Protein A and it conjugates provided either in water solution or dried powder;

Storage Conditions:

• Store at 4 ⁰C. Protect from light.

Protein A, gold labeled

Description:

Nanocs provides a variety of chemically functionalized and bioconjugated protein A with different functionality. These functional protein A products were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

• 2~7 functional groups on each protein A molecule.
• Protein A and it conjugates provided either in water solution and dried powder;

Storage Conditions:

• Store4 ⁰C, dessiccated. Protect from light.

Catalase, Fluorescein labeled

Description:

Fluorescein labeled catalase is one of Nanocs’ fluorescent catalase conjugates. Catalase is a common enzyme found in nearly all living organisms exposed to oxygen. It catalyzes the decomposition of hydrogen peroxide to water and oxygen. Catalase is a important enzyme in protetecting the cell from oxidative damage by reactive oxygen species. Fluorescein modified catalase is a green fluorescent catalase conjugates with excitation/emission wavelength around 495 nm/515 nm. These functionalized catalase conjugates were purified by size exclusion chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

• 2~7 fluorescein dyes on each catalase molecule.
• Catalase conjugates provided either in water solution or dried powder;

Storage Conditions:

• Store at 4 ⁰C. Desiccate. Protect from light.

Catalase, Cy3 labeled

Description:

Cy3 labeled catalase is one of Nanocs’ fluorescent catalase conjugates. Catalase is a common enzyme found in nearly all living organisms exposed to oxygen. It catalyzes the decomposition of hydrogen peroxide to water and oxygen. Catalase is a important enzyme in protetecting the cell from oxidative damage by reactive oxygen species. Cy3 modified catalase is a red fluorescent catalase conjugates with excitation/emission wavelength around 550 nm/570 nm. All our functionalized catalase conjugates were purified by size exclusion chromatography to ensure adequate applications both in-vitro and in-vivo.

Specifications:

• 2~7 Cy3 dyes on each catalase molecule.
• Catalase conjugates provided either in water solution or dried powder;

Storage Conditions:

• Store at 4 ⁰C. Desiccate. Protect from light.

Human serum albumin, HSA, Cy5 labeled

Description:

Nanocs provides a variety of chemically functionalized and bioconjugated Human serum albumin (HSA) with different functionality. These functional HSA products were purified by size exclusion chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

• 2~7 functional groups on each HSA molecule.
• HSA conjugates provided either in water solution or dried powder;

Storage Conditions:

• Store at 4 ⁰C. Protect from light.

Lipopolysacharides, Cy3 labeled

Lipopolysaccharide, Cy3 Conjugate

Description:

Cy3 labeled lipopolysaccharide (LPS) is one of red fluorescent LPS conjugates from Nanocs. Nanocs offers several fluorescent conjugates of lipopolysaccharides from Escherichia coli and Salmonella minnesota. LPS, also called endotoxins, are complex macromolecules present on the outer cell walls of gram-negative bacteria. Our fluoresent LPS allow researchers to follow binding, transport and cell internalization processes of LPS. We provides a variety of fluorescently labeled and other functional LPS conjugates, which include Fluorescein (FITC, FAM) LPS, Rhodamine (TRITC, TMR, TAMRA) LPS, Cy3 LPS, Cy5 LPS, Biotin LPS and many other multifunctional LPS products. If you have needs for any special LPS products not listed here, please call us and let we make for you!

Specifications:

• 1~5 Cy3 dyes on each LPS molecule.
• Lipopolysaccharide Cy3 conjugates provided either in water solution and dried powder;
• Excitation/Emission Wavelength: 550 nm/570 nm.

Storage Conditions:

• Store at 4 ⁰C. Protect from light.

Succinimidyl PEG NHS, mPEG-NHS(SC)

Description:

N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH₂) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.

Physical Properties:

• Off-white/white solid or viscous liquid depends on molecule weight;
• Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

• Store at -20 ⁰C, desiccate. NHS PEG tends to hydrolyze from moisture. Avoid frequent thaw and frozen.

Reaction Procedures:

Generally, a 10 to 50-fold molar excess of NHS PEG over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Conjugation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-free buffer at pH 7-8.5.
• PEG NHS stock solution: 100 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in conjugation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add NHS PEG stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of PEG NHS needed for optimal conjugation; Allow mixture agitates at room temperature for 30~60 min at room temperature or 2 hours at 4 ⁰C. Conjugates can be purified either by size exclusion chromatography or dialysis.

Succinimidyl PEG NHS, mPEG-NHS(SC)

Description:

N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH₂) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.

Physical Properties:

• Off-white/white solid or viscous liquid depends on molecule weight;
• Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

• Store at -20 ⁰C, desiccate. NHS PEG tends to hydrolyze from moisture. Avoid frequent thaw and frozen.

Reaction Procedures:

Generally, a 10 to 50-fold molar excess of NHS PEG over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Conjugation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-free buffer at pH 7-8.5.
• PEG NHS stock solution: 100 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in conjugation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add NHS PEG stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of PEG NHS needed for optimal conjugation; Allow mixture agitates at room temperature for 30~60 min at room temperature or 2 hours at 4 ⁰C. Conjugates can be purified either by size exclusion chromatography or dialysis.

Succinimidyl PEG NHS, mPEG-NHS(SC)

Description:

N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH₂) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.

Physical Properties:

• Off-white/white solid or viscous liquid depends on molecule weight;
• Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

• Store at -20 ⁰C, desiccate. NHS PEG tends to hydrolyze from moisture. Avoid frequent thaw and frozen.

Reaction Procedures:

Generally, a 10 to 50-fold molar excess of NHS PEG over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Conjugation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-free buffer at pH 7-8.5.
• PEG NHS stock solution: 100 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in conjugation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add NHS PEG stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of PEG NHS needed for optimal conjugation; Allow mixture agitates at room temperature for 30~60 min at room temperature or 2 hours at 4 ⁰C. Conjugates can be purified either by size exclusion chromatography or dialysis.

Succinimidyl PEG NHS, mPEG-NHS(SC)

Description:

N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH₂) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.

Physical Properties:

• Off-white/white solid or viscous liquid depends on molecule weight;
• Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

• Store at -20 ⁰C, desiccate. NHS PEG tends to hydrolyze from moisture. Avoid frequent thaw and frozen.

Reaction Procedures:

Generally, a 10 to 50-fold molar excess of NHS PEG over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Conjugation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-free buffer at pH 7-8.5.
• PEG NHS stock solution: 100 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in conjugation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add NHS PEG stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of PEG NHS needed for optimal conjugation; Allow mixture agitates at room temperature for 30~60 min at room temperature or 2 hours at 4 ⁰C. Conjugates can be purified either by size exclusion chromatography or dialysis.

Succinimidyl PEG NHS, mPEG-NHS(SC)

Description:

N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH₂) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.

Physical Properties:

• Off-white/white solid or viscous liquid depends on molecule weight;
• Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

• Store at -20 ⁰C, desiccate. NHS PEG tends to hydrolyze from moisture. Avoid frequent thaw and frozen.

Reaction Procedures:

Generally, a 10 to 50-fold molar excess of NHS PEG over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Conjugation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-free buffer at pH 7-8.5.
• PEG NHS stock solution: 100 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in conjugation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add NHS PEG stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of PEG NHS needed for optimal conjugation; Allow mixture agitates at room temperature for 30~60 min at room temperature or 2 hours at 4 ⁰C. Conjugates can be purified either by size exclusion chromatography or dialysis.

Methoxy PEG glutaric acid NHS

Description:

N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH₂) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.

Physical Properties:

• Off-white/white solid or viscous liquid depends on molecule weight;
• Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

• Store at -20 ⁰C, desiccate. NHS PEG tends to hydrolyze from moisture. Avoid frequent thaw and frozen.

Reaction Procedures:

Generally, a 10 to 50-fold molar excess of NHS PEG over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Conjugation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-free buffer at pH 7-8.5.
• PEG NHS stock solution: 100 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in conjugation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add NHS PEG stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of PEG NHS needed for optimal conjugation; Allow mixture agitates at room temperature for 30~60 min at room temperature or 2 hours at 4 ⁰C. Conjugates can be purified either by size exclusion chromatography or dialysis.

Succinimidyl acid PEG, mPEG-NHS

Description:

N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH₂) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.

Physical Properties:

• Off-white/white solid or viscous liquid depends on molecule weight;
• Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

• Store at -20 ⁰C, desiccate. NHS PEG tends to hydrolyze from moisture. Avoid frequent thaw and frozen.

Reaction Procedures:

Generally, a 10 to 50-fold molar excess of NHS PEG over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Conjugation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-free buffer at pH 7-8.5.
• PEG NHS stock solution: 100 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in conjugation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add NHS PEG stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of PEG NHS needed for optimal conjugation; Allow mixture agitates at room temperature for 30~60 min at room temperature or 2 hours at 4 ⁰C. Conjugates can be purified either by size exclusion chromatography or dialysis.