DBCO (dibenzocycolctyne) PEG Biotin derivatives can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.
Physical Properties:
White solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Copper-free Click reaction procedures:
Prepare the azide-containing reagents in reaction buffer.
Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
Purify conjugates either by dialysis or size-exclusion chromatograph.
Storage Conditions:
Store at -20 0C, dessicate. Protect from light. Avoid frequent thaw and freeze.
DBCO (dibenzocycolctyne) PEG Biotin derivatives can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.
Physical Properties:
White solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Copper-free Click reaction procedures:
Prepare the azide-containing reagents in reaction buffer.
Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
Purify conjugates either by dialysis or size-exclusion chromatograph.
Storage Conditions:
Store at -20 0C, dessicate. Protect from light. Avoid frequent thaw and freeze.
DSPE PEG DBCO (dibenzocyclooctyne) is one of Nanocs' reactive phospholipid PEG derivatives that can react with azide group via copper free Click Chemistry. DSPE (1,2-distearoyl-sn-glycero-3-phosphoethanolamine) is an 18 carbon saturated phospholipid that is covalently attached to the one terminus of PEG chain. DBCO, on the other hand, is linked to the other end of the PEG that can go Click Chemistry with azide without need any catalyst. Reaction between DBCO and azide allows the conjugation of DSPE PEG to azido functionalized molecules or materials with high efficiency. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer.
Product information:
Form: Solid;
Color: Pale yellow to yellow;
Solubility: Soluble in chloroform, warm water;
Reactive group: DBCO;
Reactive to: Azide (-N3);
Lipid type: C18 phospholipid, saturated;
Purity: >95%;
Copper-free Click reaction procedures:
Prepare the azide-containing reagents in reaction buffer.
Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
Purify conjugates either by dialysis or size-exclusion chromatograph.
Storage Conditions:
Store at -20 0C. Protect from light. Avoid frequent thaw and freeze.
DSPE PEG DBCO (dibenzocyclooctyne) is one of Nanocs' reactive phospholipid PEG derivatives that can react with azide group via copper free Click Chemistry. DSPE (1,2-distearoyl-sn-glycero-3-phosphoethanolamine) is an 18 carbon saturated phospholipid that is covalently attached to the one terminus of PEG chain. DBCO, on the other hand, is linked to the other end of the PEG that can go Click Chemistry with azide without need any catalyst. Reaction between DBCO and azide allows the conjugation of DSPE PEG to azido functionalized molecules or materials with high efficiency. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer.
Product information:
Form: Solid;
Color: Pale yellow to yellow;
Solubility: Soluble in chloroform, warm water;
Reactive group: DBCO;
Reactive to: Azide (-N3);
Lipid type: C18 phospholipid, saturated;
Purity: >95%;
Copper-free Click reaction procedures:
Prepare the azide-containing reagents in reaction buffer.
Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
Purify conjugates either by dialysis or size-exclusion chromatograph.
Storage Conditions:
Store at -20 0C. Protect from light. Avoid frequent thaw and freeze.
DBCO (dibenzocycolctyne) PEG thiol (DBCO-PEG-SH) derivatives can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.
Physical Properties:
White solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Copper-free Click reaction procedures:
Prepare the azide-containing reagents in reaction buffer.
Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
Purify conjugates either by dialysis or size-exclusion chromatograph.
Storage Conditions:
Store at -20 0C, desiccate. Protect from light. Avoid frequent thaw and freeze.
DBCO (dibenzocycolctyne) PEG Silane derivatives can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.
Physical Properties:
White solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Copper-free Click reaction procedures:
Prepare the azide-containing reagents in reaction buffer.
Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
Purify conjugates either by dialysis or size-exclusion chromatograph.
Storage Conditions:
Store at -20 0C, dessicate. Protect from light. Avoid frequent thaw and freeze.
DBCO (dibenzocycolctyne) PEG Cy5 is a near-infrared fluorescent PEG derivative that can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.
Physical Properties:
Dark blue solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Copper-free Click reaction procedures:
Prepare the azide-containing reagents in reaction buffer.
Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
Purify conjugates either by dialysis or size-exclusion chromatograph.
Storage Conditions:
Store at -20 0C, dessicate. Protect from light. Avoid frequent thaw and freeze.
DBCO (dibenzocycolctyne) PEG Cy3 is a red fluorescent PEG derivative that can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.
Physical Properties:
Dark red solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Copper-free Click reaction procedures:
Prepare the azide-containing reagents in reaction buffer.
Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
Purify conjugates either by dialysis or size-exclusion chromatograph.
Storage Conditions:
Store at -20 0C, dessicate. Protect from light. Avoid frequent thaw and freeze.
DBCO (dibenzocycolctyne) PEG Silane derivatives can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.
Physical Properties:
White solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Copper-free Click reaction procedures:
Prepare the azide-containing reagents in reaction buffer.
Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
Purify conjugates either by dialysis or size-exclusion chromatograph.
Storage Conditions:
Store at -20 0C, dessicate. Protect from light. Avoid frequent thaw and freeze.
DBCO (dibenzocycolctyne) PEG Silane derivatives can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.
Physical Properties:
White solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Copper-free Click reaction procedures:
Prepare the azide-containing reagents in reaction buffer.
Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
Purify conjugates either by dialysis or size-exclusion chromatograph.
Storage Conditions:
Store at -20 0C, dessicate. Protect from light. Avoid frequent thaw and freeze.
Dibenzocycolctyne (DBCO) PEG NHS derivatives can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.
Physical Properties:
White solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Copper-free Click reaction procedures:
Prepare the azide-containing reagents in reaction buffer.
Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
Purify conjugates either by dialysis or size-exclusion chromatograph.
Storage Conditions:
Store at -20 0C, dessicate. Protect from light. Avoid frequent thaw and freeze.
Dibenzocycolctyne (DBCO) PEG NHS derivatives can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.
Physical Properties:
White solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Copper-free Click reaction procedures:
Prepare the azide-containing reagents in reaction buffer.
Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
Purify conjugates either by dialysis or size-exclusion chromatograph.
Storage Conditions:
Store at -20 0C, dessicate. Protect from light. Avoid frequent thaw and freeze.
DBCO PEG Folic acid, also called DBCO PEG folate, is one of Nanocs' folic acid PEG derivatives that can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG folate derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. Folic acid, is also known as vitamin M, vitamin B9 or pteroyl-L-glutamic acid. Folic acid is an essential bioactive molecule for numerous biological functions. It participates in the synthesis, repairing and methylation of DNA as well as to act as a cofactor in many biological reactions. DBCO functional folic acid can be easily attached to azide functionalized molecules, particles or solid surfaces.
Physical Properties:
Yellow solid;
Soluble in DMSO;
Reactive group: DBCO;
Reactive to: Azide;
Applications: Click chemistry;
Purity: >95%;
Copper-free Click reaction procedures:
Prepare the azide-containing reagents in reaction solution.
Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
Purify conjugates either by dialysis or size-exclusion chromatograph.
Storage Conditions:
Store at -20 0C. Desicate. Protect from light. Avoid frequent thaw and freeze.
DBCO PEG NHS is one of Nanocs’ amine reactive PEG derivatives can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO (Dibenzylcyclooctyne) PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.
Physical Properties:
Off-white or light yellow solid;
Soluble in regular aqeous solution;
Functional group: DBCO;
Reactive toward: Azide (-N3):
PEG MW: 20000 Da;
Copper-free Click reaction procedures:
Prepare the azide-containing reagents in reaction buffer.
Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
Purify conjugates either by dialysis or size-exclusion chromatograph.
DBCO PEG NHS is one of Nanocs’ amine reactive PEG derivatives can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO (Dibenzylcyclooctyne) PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.
Physical Properties:
Off-white or light yellow solid;
Soluble in regular aqeous solution;
Functional group: DBCO;
Reactive toward: Azide (-N3):
PEG MW: 30000 Da;
Copper-free Click reaction procedures:
Prepare the azide-containing reagents in reaction buffer.
Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
Purify conjugates either by dialysis or size-exclusion chromatograph.
