Norbornene PEG Amine (Norbornene PEG NH2) is one of Nanocs Click Chemistry PEG Reagents that have a Norbornene group on one end of PEG chain and an amine group on the other PEG terminus. Norbornene is a strain-promoted dienophile that has been used in thiol-ene click chemistry to react with thiols. It also an excellent Click Chemistry reagent used in tetrazine-norbornene cycloaddition. Meanwhile, amine group allows conjugation of various molecules to PEG norbornene. PEG linker between Norbornene and amine offers good water solubility, better stability or flexible linker structure.
Product Specifications:
Form: Solid powder
Color: White
Solubility: Soluble in water
Reactive groups: Norbornene, amine
Reactive to: Thiol, tetrazine
Purity: >95%
Applications: Thiol-ene and tetrazine-norbornene click chemistry
Norbornene PEG Acid (Norbornene PEG COOH) is one of Nanocs Click Chemistry PEG Reagents that have a Norbornene group on one end of PEG chain and a carboxylic acid group (-COOH) on the other PEG terminus. Norbornene is a strain-promoted dienophile that has been used in thiol-ene click chemistry to react with thiols. It also an excellent Click Chemistry reagent used in tetrazine-norbornene cycloaddition. Meanwhile, COOH group allows conjugation of Norbornene group to various molecules via their amine groups. Resulted Norbornene group thus can participate various Click Chemistry reactions. PEG linker between Norbornene and COOH offers good water solubility, better stability or flexible linker structure.
Product Specifications:
Form: Solid powder
Color: White
Solubility: Soluble in water
Reactive groups: Norbornene, COOH
Reactive to: Thiol, tetrazine, amine
Purity: >95%
Applications: Thiol-ene and tetrazine-norbornene click chemistry
Norbornene PEG FITC is one of Nanocs fluorescent Click Chemistry PEG Reagents that have a Norbornene group on one end of PEG chain and a fluorescein dye molecule the other PEG terminus. Norbornene is a strain-promoted dienophile that has been used in thiol-ene click chemistry to react with thiols. It also an excellent Click Chemistry reagent used in tetrazine-norbornene cycloaddition. Meanwhile, FITC dye gives strong green fluorescence upon excited at 490 nm. Fluorescein PEG Norbornene thus can be easily detected with fluorescent instruments. PEG linker between Norbornene and FITC offers good water solubility, better stability or flexible linker structure.
Product Specifications:
Form: Solid powder
Color: Yellow
Solubility: Soluble in water
Reactive groups: Norbornene
Reactive to: Thiol, tetrazine, amine
Ex/Em wavelength: 495 nm/515 nm
Purity: >95%
Applications: Thiol-ene and tetrazine-norbornene click chemistry
Vitamin E PEG Biotin is one of Nanocs bioactive PEG derivatives that can be used for bioassay development. Vitamin E, also known as tocopherol, is one of lipid soluble vitamins that protect cell membrane from oxidative damage.
Product Specifications:
Form: Solid powder
Color: White
Solubility: Soluble in water
Functional ligand: biotin
Binding target: Avidin, streptavidin or neutravidin
NanoBright® gold nanoparticles from Nanocs have narrow size distribution yet with multiple size selection from 2 nm to 250 nm. These gold nanoparticles have spherical shape, completely soluble in water, have been used widely for the development of diagnostic probes, therapeutic agents and drug delivery vehicles. The optical and electronic properties of gold nanoparticles are tunable by changing the size, shape, surface chemistry and aggregate state. These gold nanoparticles can be further modified with different surface coatings and functional groups to render them with unique chemical and biological reactivity. Nanocs surface modified gold particles provide more choices for in-vitro or in-vivo applications of those nanoparticles. Customer conjugation and modification is available upon request.
NanoBright® Gold Nanoparticle Properties:
Orange, red or purple aqueous soution;
Concentration: 0.01% (w/v) based on gold;
Gold nanoparticle Concentration and other properties:
Particle Size (nm)
Particle Conc. (Particles/mL)
SPR Wavelength (nm)
Particle Size (nm)
Particle Conc. (Particles/mL)
SPR Wavelength (nm)
2
1.5x10E14
Not measured
40
9.0x10E10
~530
3
1.2x10E14
512~515
50
4.5x10E10
~535
5
5.0x10E13
515~520
60
3.1x10E10
~540
10
5.7x10E12
515~520
80
2.6x10E10
~553
15
1.4x10E12
517~522
100
1.1x10E10
~572
20
7.0x10E11
525
150
5.6x10E9
Not measured
30
2.0x10E11
527
200
1.7x10E9
Not measured
Gold Nanoparticle Storage Conditions:
Store at 4 0C. DO NOT FREEZE.
Selected publications using Nanocs NanoBright® gold nanoparticles:
Hyungsoon Im et al, Label-free detection and molecular profiling of exosomes with a nano-plasmonic sensor. Nature Biotech 32, 490-495 (2014)
Yongkun Yang, Peter Burkhard: Encapsulation of gold nanoparticles into self-assembling protein nanoparticles. J of Nanobiotech 2012, 10:42.
Stacey L. et al. Protein-Ligand Binding Investigated by a Single Nanoparticle TERS Approach. Chem Commun 2011; 47(7): 2065–2067
Yoshiaki Maeda, et al, Noncovalent Immobilization of Streptavidin on In Vitro- and In Vivo-Biotinylated Bacterial Magnetic Particles. Appl. Environ. Microbiol. 2008 vol. 74 no. 16 5139-5145
Peptide gold cluster probe (Au26P9) provided by Nanocs is a cell targeting probe consisted of 26 gold (Au) atoms and 9 peptides (peptide sequence: H2N-CCYHWKHLHNTKTEL-COOH). This gold nanocluster peptide conjugate is a useful dual functional probe that can be detected by both fluorescent and mass spectrometry instruments.Au26P9 probe can target and quantify proteins on cell membrane and/or tissue sections base on its verstile peptide sequence. Proteins on cell membrance can be illuminated via intrisic fluorescence of gold clusters and be pricisely quantified by counting of Au via ICP-MS at single cell level. Gold cluster peptide probe can label many different proteins including MMPS, Integrins, EGFR, GPx-1, Cadherin and other proteins via the flexible design of pepdies conjugated to gold cluster.
Product Specifications:
Form: Liquid;
Color: Light yellow;
Gold atom numbers: 26
Peptide sequence: H2N-CCYHWKHLHNTKTEL-COOH
Concentration: 400 uM;
Excitation: 540 nm;
Emission: 640 nm;
Formular: Au26P9;
Storage Conditions:
Store at 4-8 0C. Protect from light. DO NOT FREEZE.
Please note that peptide sequence and the number of Au atoms can be specifically selected and synthesized with customer request according to your unique cell or tissue analysis requirement.
References:
Zhang, X.; Liu, R.; Yuan, Q.; Gao, F.; Li, J.; Zhang, Y.; Zhao, Y.; Chai, Z.; Gao, L.; Gao, X., The Precise Diagnosis of Cancer Invasion/Metastasis via 2D Laser Ablation Mass Mapping of Metalloproteinase in Primary Cancer Tissue. ACS Nano 2018, 12, 11139-11151.
Zhang, X.; Liu, R.; Shu, Q.; Yuan, Q.; Xing, G.; Gao, X., Quantitative Analysis of Multiple Proteins of Different Invasive Tumor Cell Lines at the Same Single-Cell Level. Small 2018, 14, 1703684.
Zhai, J.; Wang, Y.; Xu, C.; Zheng, L.; Wang, M.; Feng, W.; Gao, L.; Zhao, L.; Liu, R.; Gao, F.; Zhao, Y.; Chai, Z.; Gao, X., Facile approach to observe and quantify the alpha(IIb)beta3 integrin on a single-cell. Anal. Chem. 2015, 87, 2546-2549.
Liu, R.; Zhai, J.; Liu, L.; Wang, Y.; Wei, Y.; Jiang, X.; Gao, L.; Zhu, H.; Zhao, Y.; Chai, Z.; Gao, X., Spatially marking and quantitatively counting membrane immunoglobulin M in live cells via Ag cluster-aptamer probes. Chem. Commun. 2014, 50, 3560-3563.
Nanocs provides various functionalized superparamagnetic particles that can be used for biomolecule separation, imaging and diagnostic application. Our small sized paramagnetic nanoparticles, with diameter in 25~100 nm, are suitable for fluorescent imaging, cellular and biomolecular labeling and magnetic sensing. Because of their small size, this type of magnetic nanoparticles cannot extracted with regular magnets. Our large size magnetic particles, with diameter 100 nm or above, can be exacted with magnets and therefore, can be used for cellular and biomolecule separation. Customer conjugation and modification is available upon request.
Physical Properties:
1% suspension in aqueous solution, other concentration available upon request;
Similar names: Dextran NHS, Succinimidyl NHS Dextran
Description:
NHS activated dextran (Dextran-NHS) is one of Nanocs chemical reactive dextran products that can react with primary amine groups under alkaline pH. NHS reacts rapidly with primary amine group at pH 8-10 to form a stable amide bond. NHS activated dextran can thus react rapidly with proteins, peptides or small molecules.
Dextrans are naturally synthesized polysaccharides with good water solubility, low toxicity and varied molecular weights. Dextrans modified with dyes and other biomolecules have been successfully used as long-term tracers for live cells. Nanocs also provides customized dextran products for your special needs.
Nanocs provides a variety of chemically functionalized and bioconjugated dextran products with molecular weight ranged from 3,000 Da to 2,000 KDa. These functional dextrans were purified by size-exclusion chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.
Similar names: Dextran NHS, Succinimidyl NHS Dextran
Description:
NHS activated dextran (Dextran-NHS) is one of Nanocs chemical reactive dextran products that can react with primary amine groups under alkaline pH. NHS reacts rapidly with primary amine group at pH 8-10 to form a stable amide bond. NHS activated dextran can thus react rapidly with proteins, peptides or small molecules.
Dextrans are naturally synthesized polysaccharides with good water solubility, low toxicity and varied molecular weights. Dextrans modified with dyes and other biomolecules have been successfully used as long-term tracers for live cells. Nanocs also provides customized dextran products for your special needs.
Nanocs provides a variety of chemically functionalized and bioconjugated dextran products with molecular weight ranged from 3,000 Da to 2,000 KDa. These functional dextrans were purified by size-exclusion chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.
