Insulin, Cy5 labeled

Synonym: Cy5-Insulin, Insulin-Cy5, Insulin Cy5 conjugate, Cy5 Insulin conjugate.

Description:

Cy5-Insulin is one of Nanocs fluorescent Insulin conjugates that are labeled with near infrared cyanine dyes. Insulin is a protein secreted by beta cells of the pancreas. Primary function of Insulin is to regulate carbohydrate and fat metabilismin the body. Human insulin has 51 amino acids with a molecular weigth of 5806 Da. It is a dimmer of two peptide chains connected by disulfide bonds. Cy5 is a bright near-infrared fluorescent dye that has excitation/emission wavelength at around 650 nm/670 nm.  Because of its excellent tissue penetration capability, Cy5 labeled Insulin can be used for in-viov imaging. 

Nanocs provides a variety of chemically functionalized and bioconjugated Insulin with different functionality, including biotin Insulin, fluorescein labeled insulin, rhodamine, Cy3, Cy5, Cy5.5, Cy7, Cy7.5 and many other fluorophore labeled insulin conjugate. These functional Insulin products were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Product Specifications:

• Insulin type: Recommbinant human insulin.
• 1~2 labeled dyes on each Insulin molecule.
• Ex/Em wavelength: 650 nm/670 nm.
• Form: Lyophilized powder or buffered solution.
• Color: Blue.
• Applications: Fluorescent imaging or fluorescent detection.

Storage Conditions:

• Store at 4 ⁰C or lower. Protect from light.

Insulin, Biotin labeled

Description:

Nanocs provides a variety of chemically functionalized and bioconjugated Insulin with different functionality, including biotin Insulin, fluorescein labeled insulin, rhodamine, Cy3, Cy5, Cy5.5 and many other fluorophore labeled insulin conjugate. These functional Insulin products were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

• Insulin type: Recommbinant human insulin.
• 1~2 labeled groups on each Insulin molecule.
• Insulin conjugates provided either in water solution and dried powder;

Storage Conditions:

• Store at 4 ⁰C, dessiccate. Protect from light.

Insulin, Alexa fluor 546 labeled

Description:

Nanocs provides a variety of chemically functionalized and bioconjugated Insulin with different functionality, including biotin Insulin, fluorescein labeled insulin, rhodamine, Cy3, Cy5, Cy5.5 and many other fluorophore labeled insulin conjugate. These functional Insulin products were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

• Insulin type: Recommbinant human insulin.
• 1~2 labeled groups on each Insulin molecule.
• Insulin conjugates provided either in water solution and dried powder;

Storage Conditions:

• Store at 4 ⁰C, dessiccate. Protect from light.

Insulin, Alexa fluor 647 labeled

Description:

Nanocs provides a variety of chemically functionalized and bioconjugated Insulin with different functionality, including biotin Insulin, fluorescein labeled insulin, rhodamine, Cy3, Cy5, Cy5.5 and many other fluorophore labeled insulin conjugate. These functional Insulin products were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

• Insulin type: Recommbinant human insulin.
• 1~2 labeled groups on each Insulin molecule.
• Insulin conjugates provided either in water solution and dried powder;

Storage Conditions:

• Store at 4 ⁰C, dessiccate. Protect from light.

Insulin, Alexa fluor 680 labeled

Description:

Nanocs provides a variety of chemically functionalized and bioconjugated Insulin with different functionality, including biotin Insulin, fluorescein labeled insulin, rhodamine, Cy3, Cy5, Cy5.5 and many other fluorophore labeled insulin conjugate. These functional Insulin products were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

• Insulin type: Recommbinant human insulin.
• 1~2 labeled groups on each Insulin molecule.
• Insulin conjugates provided either in water solution and dried powder;

Storage Conditions:

• Store at 4 ⁰C, dessiccate. Protect from light.

Fluorescein NHS, 5(6)-Carboxyfluorescein N-hydroxysuccinimide Ester

5(6)-Carboxyfluorescein

Rhodamine B isothiocyanate (RITC)

Pyrenyl butyrate NHS

7-methoxycoumarin NHS

7-diethylaminocoumarin NHS

SPDP (Succinimidyl 3-[2-pyridyldithio] propionate)

SPDP Crosslinker Description:

Nanocs SnapLink^TM SPDP crosslinker, N–Succinimidyl 3-(2-pyridyldithio) propionic acid, also called N-succinimidyl 3-(2-pyridyldithio)-propionate, or OPSS-NHS, is a short-length crosslinker for amine to sulfhydryl (thiol) conjugation via its NHS-ester and pyridyldithiol reactive groups that form cleavable disulfide bonds with free thiol groups. Nanocs LC-SPDP is a long-length crossliker for amine to sulfhydryl conjugation via its NHS-ester and pyridyldithiol reactive groups that form cleavable disulfide bonds with free thiol groups.

In addition to above hydrophobic SPDP crosslinker, Nanocs also offer water-soluble SPDP crosslinker either with sulfonated NHS (Sulfo-SPDP) or Pegylated SPDP crosslinker, such as our PEG OPSS products. These SPDP crosslinkers are heterobifunctional and thiol cleavable crosslinkers.

SPDP Crosslinker Physical Properties:

• Appearance: Off-white/white solid;
• Reactive groups: NHS ester and pyridyldithiol;
• Reactive towards: amino (amine) and sulfhydryl (thiol) groups;
• SPDP crosslinker is membrane permeable;
• Solubility: Soluble in DMF or DMSO;

SPDP Applications:

• Adding thiol/sulfhydryl groups to proteins, antibodies or other molecules by reacting with amine groups;
• Reversible crosslinking of proteins or other molecules;

SPDP Storage Conditions:

• Store at -20 ⁰C, desicate. Avoid frequent thaw and freeze.

SMCC/Maleimidocyclohexane NHS

Name: Succinimidyl 4-(maleimidomethyl)cyclohexane-1-carboxylate, SMCC

Synonym: 4-(N-Maleimidomethyl)cyclohexanecarboxylic acid N-hydroxysuccinimide ester

Description about SMCC:

SMCC, also called Succinimidyl cyclohexane Maleimide, is one of  Nanocs' amine and sulfhydryl reactive crosslinkers. This crossliner is non-cleavable and membrane permeable. It contains an amine reactive NHS ester and a sulfhydryl reactive maleimide group. Compared to other maleimide NHS ester, SMCC has higher stability in aqueous solution because of its cyclohexane bridge. NHS esters react with primary amines at pH 7-9 while the maleimide group reacts with available thiol or sulfhydryl at pH 6.5~7.5 to form stable thioether bonds.

Physical Properties of SMCC:

• Appearance: Off-white/white solid;
• Molecular formula: C16H18N2O6;
• Molecular weight: 334.32;
• Spacer arm length: 8.3 angstrom, 9 atoms
• CAS number: 64987-85-5;
• Purity: >95%
• Soluble in DMF or DMSO;

Storage Conditions of SMCC:

• SMCC should be stored at -20 ⁰C or lower. Desiccate. Avoid frequent thaw and freeze.

SPDP-Hydrazide (PDPH)

SPDP Crosslinker Description:

Nanocs SnapLink^TM SPDP crosslinker, N–Succinimidyl 3-(2-pyridyldithio) propionic acid, also called N-succinimidyl 3-(2-pyridyldithio)-propionate, or OPSS-NHS, is a short-length crosslinker for amine to sulfhydryl (thiol) conjugation via its NHS-ester and pyridyldithiol reactive groups that form cleavable disulfide bonds with free thiol groups. Nanocs LC-SPDP is a long-length crossliker for amine to sulfhydryl conjugation via its NHS-ester and pyridyldithiol reactive groups that form cleavable disulfide bonds with free thiol groups.

In addition to above hydrophobic SPDP crosslinker, Nanocs also offer water-soluble SPDP crosslinker either with sulfonated NHS (Sulfo-SPDP) or Pegylated SPDP crosslinker, such as our PEG OPSS products. These SPDP crosslinkers are heterobifunctional and thiol cleavable crosslinkers.

SPDP Crosslinker Physical Properties:

• Appearance: Off-white/white solid;
• Reactive groups: NHS ester and pyridyldithiol;
• Reactive towards: amino (amine) and sulfhydryl (thiol) groups;
• SPDP crosslinker is membrane permeable;
• Solubility: Soluble in DMF or DMSO;

SPDP Applications:

• Adding thiol/sulfhydryl groups to proteins, antibodies or other molecules by reacting with amine groups;
• Reversible crosslinking of proteins or other molecules;

SPDP Storage Conditions:

• Store at -20 ⁰C, desicate. Avoid frequent thaw and freeze.

N-succinimidyl S-acetylthioacetate (SATA)

N-succinimidyl S-acetylthiopropionate (SATP)

Pyridine dithioethylamine (PDA)

Description:

Pyridine dithioethylamine (PDA) is a sulfhydryl amination reagent. Pyridyl disulfide group can react with sulfhydryl group to form cleavable disulfide bond. Remaining primary amine group can be used to react with a variety groups. Released 2-thiopyridone group can be quantitatively measured at 343 nm. .

Physical Properties:

• Appearance: Off-white/white solid;
• Purity: >95%
• Solubility: Soluble in water, DMF or DMSO;

Storage Conditions:

• Store at -20 ⁰C, dessiccated. Avoid frequent thaw and freeze.

Succinimidyl iodoacetate (SIA)

Succinimidyl bromoacetate (SBA)

Maleimidocyclohexane acid NHS/SMCC

Name: Succinimidyl 4-(maleimidomethyl)cyclohexane-1-carboxylate, SMCC

Synonym: 4-(N-Maleimidomethyl)cyclohexanecarboxylic acid N-hydroxysuccinimide ester

Description about SMCC:

SMCC, also called Succinimidyl cyclohexane Maleimide, is one of  Nanocs' amine and sulfhydryl reactive crosslinkers. This crossliner is non-cleavable and membrane permeable. It contains an amine reactive NHS ester and a sulfhydryl reactive maleimide group. Compared to other maleimide NHS ester, SMCC has higher stability in aqueous solution because of its cyclohexane bridge. NHS esters react with primary amines at pH 7-9 while the maleimide group reacts with available thiol or sulfhydryl at pH 6.5~7.5 to form stable thioether bonds.

Physical Properties of SMCC:

• Appearance: Off-white/white solid;
• Molecular formula: C16H18N2O6;
• Molecular weight: 334.32;
• Spacer arm length: 8.3 angstrom, 9 atoms
• CAS number: 64987-85-5;
• Purity: >95%
• Soluble in DMF or DMSO;

Storage Conditions of SMCC:

• SMCC should be stored at -20 ⁰C or lower. Desiccate. Avoid frequent thaw and freeze.