Monarch Plasmid DNA Elution Buffer is designed for use with the Monarch Plasmid Miniprep Kit (T1010S/L), Monarch DNA Gel Extraction Kit (T1020S/L) and Monarch PCR & DNA Cleanup Kit (5 µg) (T1030S/L). This slightly alkaline buffer solubilizes and releases the DNA from the column matrix.

Learn More About Monarch

• NEBMonarch.com
• Optimize your results with our unique column design
• Enhance your DNA purification experience
• Feel good about choosing Monarch
• Choose Monarch kits for pure value

Storage Temperature

25°C 

Companion Products

• Monarch™ Plasmid Miniprep Kit 
• Monarch™ DNA Gel Extraction Kit
• Monarch™ PCR & DNA Cleanup Kit (5 μg)
• Monarch™ Plasmid Miniprep Columns
• Monarch™ DNA Cleanup Columns (5 μg)

Monarch DNA Cleanup Binding Buffer is designed for use with the Monarch PCR & DNA Cleanup Kit (5 µg) (T1030S/L). This buffer is used to dilute the DNA sample and ensure it is compatible for loading onto the column matrix.

Learn More About Monarch

• NEBMonarch.com
• Optimize your results with our unique column design
• Enhance your DNA purification experience
• Feel good about choosing Monarch
• Choose Monarch kits for pure value

Storage Temperature

25°C 

Companion Products

• Monarch™ PCR & DNA Cleanup Kit (5 μg)
• Monarch™ DNA Cleanup Columns (5 μg)
• Monarch™ DNA Elution Buffer
• Monarch™ DNA Wash Buffer

Monarch DNA Wash Buffer is designed for use with the Monarch DNA Gel Extraction Kit (T1020S/L) and the Monarch PCR & DNA Cleanup Kit (5 µg) (T1030S/L). This buffer ensures enzymes, short primers (≤ 40 nt), detergents and other low-molecular weight reaction components (e.g., nucleotides, DMSO, betaine) are removed.

Learn More About Monarch

• NEBMonarch.com
• Optimize your results with our unique column design
• Enhance your DNA purification experience
• Feel good about choosing Monarch
• Choose Monarch kits for pure value

Storage Temperature

25°C 

Companion Products

• Monarch™ PCR & DNA Cleanup Kit (5 μg)
• Monarch™ DNA Cleanup Columns (5 μg)
• Monarch™ DNA Elution Buffer
• Monarch™ Gel Dissolving Buffer

Monarch DNA Cleanup Columns (5 μg), supplied with both the Monarch DNA Gel Extraction Kit and the Monarch PCR & DNA Cleanup Kit (5 μg), have been custom designed to deliver excellent performance for the purification of DNA from agarose gels and enzymatic reactions, including PCR. Monarch columns are designed without a frit (which is commonly used in purification columns to hold the membrane in place), eliminating buffer retention and the risk of carryover contamination. The tapered design of the DNA Cleanup Column enables elution in as little as 6 μl. Monarch columns are suitable for use with centrifugation and vacuum purification protocols. The columns fit snugly into the collection tubes to enable easy handling. Additionally, Monarch columns use less plastic than conventional columns, reducing their environmental footprint without compromising performance. 

Monarch DNA Cleanup Column Design 

Monarch columns are designed for performance

Monarch columns are designed without a frit, which eliminates buffer retention and the risk of carryover contamination, providing fast, worry-free DNA purification.

Learn More About Monarch

• NEBMonarch.com
• Optimize your results with our unique column design
• Enhance your DNA purification experience
• Feel good about choosing Monarch
• Choose Monarch kits for pure value

Highlights

• No buffer retention/risk of carryover contamination
• Low elution volumes (≥ 6 μl)
• Snug fit into collection tubes for easy handling
• Made with less plastic than conventional columns

Storage Temperature

25°C

Binding Capacity

5 μg